Alexa Fluor® 488 anti-rat IgM Antibody

Pricing & Availability
Clone
MRM-47 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG
MRM-47_A647_IgM_Antibody_1_062819
LEW/s rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 488(filled histogram), or rat IgM isotype control(open histogram).
  • MRM-47_A647_IgM_Antibody_1_062819
    LEW/s rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 488(filled histogram), or rat IgM isotype control(open histogram).
  • MRM-47_A647_IgM_Antibody_2_062819
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective.
  • MRM-47_A647_IgM_Antibody_3_062819
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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408907 25 µg $101.00
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408908 100 µg $229.00
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Description

The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Product Citations
  1. Duarte C, et al. 2022. J Cell Mol Med. 26:2841. PubMed
RRID
AB_2814389 (BioLegend Cat. No. 408907)
AB_2814389 (BioLegend Cat. No. 408908)
Go To Top Version: 1    Revision Date: 06/28/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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