- Clone
- 11D8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histone-H3, H3
- Isotype
- Mouse IgG2b, κ
-
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl Antibody (Negative, Cat. No. 400362) or (B-D) Alexa Fluor® 594 Histone H3 Phospho (Ser10) Antibody (Clone 11D8) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/400.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 650809 | 25 µg | $159.00 | |||
| 650810 | 100 µg | $370.00 | |||
Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Modified synthetic peptide conjugated to KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 2 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.
- Product Citations
-
- RRID
-
AB_2801122 (BioLegend Cat. No. 650809)
AB_2801122 (BioLegend Cat. No. 650810)
Antigen Details
- Structure
- H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
- Distribution
-
Nucleus
- Function
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
- Interaction
- Two molecules of H3 form a heterotetramer with two molecules of H4.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
4. Hans F, et al. 2001. Oncogene 20:3021. - Regulation
- H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
- Gene ID
- 8290 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Other Formats
View All Histone Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-Histone H3 Phospho (Ser10) | 11D8 | ICFC |
| Purified anti-Histone H3 Phospho (Ser10) | 11D8 | WB |
| Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10) | 11D8 | ICFC |
| Alexa Fluor® 647 anti-Histone H3 Phospho (Ser10) | 11D8 | ICFC |
| Alexa Fluor® 594 anti-Histone H3 Phospho (Ser10) | 11D8 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-Histone H3 Phospho (Ser10)
HeLa cells were treated with 20 µM of Nocodazole for 24 hour... -
Purified anti-Histone H3 Phospho (Ser10)
293T cell extracts were resolved by electrophoresis, transfe... 
Exponentially growing Hela cells were stained with purified ... 
-
Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10)
Hela cells were treated with 20 µM of Nocodazole for 24 hour... -
Alexa Fluor® 647 anti-Histone H3 Phospho (Ser10)
HeLa cells, which were treated with 20 µM of Nocodazole for ... -
Alexa Fluor® 594 anti-Histone H3 Phospho (Ser10)
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 ...