Alexa Fluor® 594 anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
HCD14_AF594_CD14_Antibody_IF_030714
Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA) and then stained with 10 µg/ml anti-human CD14 (clone HCD14) Alexa Fluor® 594 (red), 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green), and 10 µg/ml CD8 (clone RPA-T8) Alexa Fluor® 647 (yellow) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • HCD14_AF594_CD14_Antibody_IF_030714
    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA) and then stained with 10 µg/ml anti-human CD14 (clone HCD14) Alexa Fluor® 594 (red), 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green), and 10 µg/ml CD8 (clone RPA-T8) Alexa Fluor® 647 (yellow) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • HCD14_AF594_FC_111214
    Human peripheral blood monocytes were stained with CD14 (clone HCD14) Alexa Flour® 594 (filled histogram) or mouse IgG1, κ Alexa Flour® 594 isotype control (open histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with the Yellow-Green Laser (561 nm).
Compare all formats See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability
325630 100 µg $265.00
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. McCoy C, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005279. PubMed
  2. Burel JG, et al. 2019. Elife. 8. PubMed
  3. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  4. Bzymek R, et al. 2016. Sci Rep. 6: 25016: . PubMed
RRID
AB_2563225 (BioLegend Cat. No. 325630)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 4    Revision Date: 10/28/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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