PE/Cyanine7 anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
HCD14_PECy7_100507
Human peripheral blood monocytes stained with HCD14 PE/Cyanine7
  • HCD14_PECy7_100507
    Human peripheral blood monocytes stained with HCD14 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
325617 25 tests $153.00
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325618 100 tests $311.00
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Wang H, et al. 2020. Cell Stem Cell. 28(3):535-549.e8. PubMed
  2. Miggitsch C, et al. 2019. EBioMedicine. 46:387. PubMed
  3. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  4. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  5. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
  6. Lucas C, et al. 2020. Nature. 584:463. PubMed
  7. Fletcher H, et al. 2016. Nat Commun. 7: 11290. PubMed
  8. Sim J, et al. 2019. MAbs. 11:1036. PubMed
  9. Vetsika EK, et al. 2021. Cancers (Basel). 13:. PubMed
  10. Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
  11. Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed
  12. Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
  13. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  14. Gillissen MA, et al. 2018. Blood. 131:131. PubMed
  15. Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed
  16. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  17. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  18. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  19. Koga H, et al. 2018. Front Immunol. 1.456944444. PubMed
  20. Haller PM, et al. 2020. Biomedicines. 8:. PubMed
  21. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  22. Rotolo A et al. 2018. Cancer cell. 34(4):596-610 . PubMed
  23. Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed
  24. Ball M, et al. 2016. PLoS One. 11: 0149600. PubMed
  25. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
  26. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  27. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  28. Headland S, et al. 2014. Sci Rep. 4:5237. PubMed
  29. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
RRID
AB_830691 (BioLegend Cat. No. 325617)
AB_830691 (BioLegend Cat. No. 325618)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 2    Revision Date: 07/13/2015

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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