Alexa Fluor® 594 anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
G10F5_A594_CD66b_Antibody_032718
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered Saline 1X (pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 (Cat. No. 925501) twice for 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluor® 647 anti-CD45RA (clone HI100) antibody (green), Alexa Fluor® 594 anti-CD66b (clone G10F5) antibody (red) and Brilliant Violet 421™ anti-CD45RO antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • G10F5_A594_CD66b_Antibody_032718
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered Saline 1X (pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 (Cat. No. 925501) twice for 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluor® 647 anti-CD45RA (clone HI100) antibody (green), Alexa Fluor® 594 anti-CD66b (clone G10F5) antibody (red) and Brilliant Violet 421™ anti-CD45RO antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
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305123 25 µg $159.00
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305124 100 µg $382.00
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed
RRID
AB_2728242 (BioLegend Cat. No. 305123)
AB_2728242 (BioLegend Cat. No. 305124)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org
Go To Top Version: 1    Revision Date: 03/29/2018

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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