C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse GL7 (clone GL7) purified, anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) and anti-mouse CD3 (clone 17A2) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Alexa Fluor® 594 (red) for 2 hours at room temperature. Staining of secondary (MRM-47) only showed no non-specific binding. The image was captured by 10X objective.
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse GL7 (clone GL7) purified, anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) and anti-mouse CD3 (clone 17A2) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Alexa Fluor® 594 (red) for 2 hours at room temperature. Staining of secondary (MRM-47) only showed no non-specific binding. The image was captured by 10X objective.
The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.25 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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