Alexa Fluor® 647 anti-α Smooth Muscle Actin Antibody

Pricing & Availability
Clone
1A4 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
Isotype
Mouse IgG2a, κ
A. 1A4_A647_Alpha-Smooth_IHC-P_061924
IHC staining of Alexa Fluor® 647 anti-α Smooth Muscle Actin (clone 1A4) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with Alexa Fluor® 647 anti-α Smooth Muscle Actin (clone 1A4) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panel B) or 40X (panel A) objective. Scale bar: 50 µm
  • A. 1A4_A647_Alpha-Smooth_IHC-P_061924
    IHC staining of Alexa Fluor® 647 anti-α Smooth Muscle Actin (clone 1A4) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with Alexa Fluor® 647 anti-α Smooth Muscle Actin (clone 1A4) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panel B) or 40X (panel A) objective. Scale bar: 50 µm
  • B. 1A4_A647_Alpha-Smooth_ICC_061924
    NIH/3T3 cells were treated with (panels A and B) or without 5 ng/mL of Recombinant Mouse TGF-β1 (Cat. No. 763104) (panels C and D) for 48 hours at 37°C, fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-α Smooth Muscle Actin (clone 1A4) (panels A and C). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were merged (panels B and D). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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614855 25 µg $175.00
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614856 100 µg $435.00
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Description

Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins expressed in mammals. α-smooth muscle actin is widely expressed in contractile cells, most notably the mural cells that line arteries and arterioles. Overexpression of α-smooth muscle actin is a hallmark of fibrosis, a thickening or scarring of damaged tissue.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of  5.0 - 10.0 µg/mL is suggested.  For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For use in immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissues, we recommend heat-induced antigen retrieval with either Tris-EDTA pH 9.0 or Citrate Buffer, 10X (Cat. No. 420902).

For use in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X.

Previously Covance catalog# MMS-466S.

RRID
AB_3662309 (BioLegend Cat. No. 614855)
AB_3662309 (BioLegend Cat. No. 614856)

Antigen Details

Structure
α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD.
Distribution

Cytoplasmic, cytoskeleton

Function
Cell motility
Interaction
α smooth muscle actins bind to myosins to produce motive force
Cell Type
Mesenchymal Stem Cells, Monocytes
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References
  1. Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83.
  2. Rizeq MN, et al. 1994. Hum Pathol. 25:671-7.
  3. Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42
  4. Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74.
  5. Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613.
  6. Skalli O, et al. 1986. J Cell Biol. 103:2787-96.
Gene ID
59 View all products for this Gene ID
UniProt
View information about alpha Smooth Muscle Actin on UniProt.org
Go To Top Version: 1    Revision Date: 06/20/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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