- Clone
- 1A4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Actin, aortic smooth muscle, alpha 2 actin, alpha-actin-2, alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
- Previously
-
Covance Catalog# MMS-466S
- Isotype
- Mouse IgG2a
-
Immunohistochemical staining of Smooth Muscle Actin (1A4) Monoclonal Antibody, on formalin fixed paraffin embedded human breast tissue at 1/50 dilution.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 904601 | 250 µL | $352.00 | |||
Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins being expressed in mammals and birds. The actin protein consists of six isoforms, varying in their amino acid sequence, but all having the same molecular mass of 42 kD. The isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. The N-terminal region appears to be a major antigenic region. There are different α isoforms specific for muscle tissues, i.e. skeletal muscle α cardiac muscle α and smooth muscle α respectively. The β- and γ-actins may be present in muscle cells as well as most other cell types in the body, including non-muscle cells.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, Cow, Chicken
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against the N-terminal synthetic decapeptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
- Formulation
- Cell Culture Supernatant (dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3).
- Preparation
- Tissue Culture Supernatant
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-P, ICC, WB
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100*
• IHC: 1:50-1:100**
Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is recommended. Optimal results are obtained with 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0. Pre-treatment of tissues with proteinase-K was found destructive of the epitope. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
Frozen sections and cell preparations: The antibody can also be used for labelling acetone-fixed, frozen sections. - Application Notes
-
This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC).
*Predicted MW = 42 kD
**Reccomend 20 minute heat-induced epitope retrieval in 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0, and 30 minute incubation of primary antibody at room temperature. -
Application References
(PubMed link indicates BioLegend citation) -
- Brennan PA, et al. 2000. J Oral Pathol Med. 29:279.
- Rizeq MN, et al. 1994. Hum Pathol. 25:671.
- Schmitt-Graff A, et al. 1991. Am J Pathol. 138:1233.
- Roholl PJM, et al 1990. Hum Pathol. 21:1269.
- Alberts B, et al. 1989. Eds. Molecular biology of the cell. 2:613.
- Skalli O, et al. 1986. J Cell Biol. 103:2787.
- Product Citations
-
- RRID
-
AB_2565041 (BioLegend Cat. No. 904601)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Gene ID
- 59 View all products for this Gene ID
- UniProt
- View information about Alpha Smooth Muscle Actin on UniProt.org
Other Formats
View All Alpha Smooth Muscle Actin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-Alpha Smooth Muscle Actin | 1A4 | IHC-P,ICC,WB |
| Purified anti-α Smooth Muscle Actin | 1A4 | IHC-P,ICC,WB |
| Alexa Fluor® 488 anti-α Smooth Muscle Actin | 1A4 | IHC-P,ICC |
| Alexa Fluor® 647 anti-α Smooth Muscle Actin | 1A4 | IHC-P,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-Alpha Smooth Muscle Actin
Immunohistochemical staining of Smooth Muscle Actin (1A4) Mo... -
Purified anti-α Smooth Muscle Actin
IHC staining of purified anti-α smooth muscle actin (clone 1... 
HeLa cells were fixed with ice-cold methanol and blocked wit... 
Whole cell extracts (15 µg total protein) from the indicated... -
Alexa Fluor® 488 anti-α Smooth Muscle Actin
IHC staining of Alexa Fluor® 488 anti-α Smooth Muscle Actin ... 
NIH/3T3 cells were treated with (panels A and B) or without ... -
Alexa Fluor® 647 anti-α Smooth Muscle Actin
IHC staining of Alexa Fluor® 647 anti-α Smooth Muscle Actin ... 
NIH/3T3 cells were treated with (panels A and B) or without ...