Alexa Fluor® 647 anti-human Cadherin 11 Antibody

Pricing & Availability
Clone
16G5 (See other available formats)
Regulatory Status
RUO
Other Names
OSF-4, Osteoblast cadherin, OB-cadherin, CDH11, CDHOB, CAD11
Isotype
Mouse IgG1, κ
16G5_A647_Cadherin-11_Antibody_IHC_101217
Human paraffin-embedded ovary tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Tween 20 in PBS and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluro®647 anti-cadherin 11 (Clone 16G5) antibody (red) and Brilliant Violet 421™ anti-Nestin Antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 16G5_A647_Cadherin-11_Antibody_IHC_101217
    Human paraffin-embedded ovary tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Tween 20 in PBS and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluro®647 anti-cadherin 11 (Clone 16G5) antibody (red) and Brilliant Violet 421™ anti-Nestin Antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
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368713 25 µg $147.00
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Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 11 is a type I transmembrane glycoprotein. It is a type II (atypical) cadherin, based on its lack of a HAV cell adhesion recognition sequence that is specific to type I cadherins. The expression of this particular cadherin in osteoblastic cell lines along with its upregulation during differentiation suggests a specific function in bone development and maintenance. This cadherin is also expressed in some cancer cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

RRID
AB_2721403 (BioLegend Cat. No. 368713)

Antigen Details

Structure
Type I single pass transmembrane glycoprotein.
Distribution

Osteoblasts, neuroblasts, and some cancer cells.

Function
Cell adhesion.
Interaction
Cadherin 2 (CDH2).
Ligand/Receptor
Calcium.
Cell Type
Osteoclasts
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Wheelock MJ, Johnson KR. 2003. Annu. Rev. Cell Dev. Biol. 19:207-35.
2. Christofori G, et al. 2003. EMBO. J. 22:2318.
3. Okazaki M, et al. 1994. J. Biol. Chem. 269:12092.

Gene ID
1009 View all products for this Gene ID
UniProt
View information about Cadherin 11 on UniProt.org
Go To Top Version: 1    Revision Date: 10/13/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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