Alexa Fluor® 647 anti-human CD209 (DC-SIGN) Antibody

Pricing & Availability
Clone
9E9A8 (See other available formats)
Regulatory Status
RUO
Other Names
Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin
Isotype
Mouse IgG2a, κ
1_9E9A8_A647_090808.jpg
Human monocytes-derived dendritic cells were stained with CD209 (clone 9E9A8) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 1_9E9A8_A647_090808.jpg
    Human monocytes-derived dendritic cells were stained with CD209 (clone 9E9A8) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 2_9E9A8_AF647_CD209_Antibody_IF_011414
    Human peripheral blood mononuclear cell (PBMC) derived dendritic cells were fixed with 1% paraformaldehyde (PFA) and then stained with 10 µg/ml of CD209 (DC-SIGN) (clone 9E9A8) Alexa Fluor® 647 for 30 minutes at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured by 40X objective.
  • 3_12_Human_LN_CD3_DC-SIGN
    Confocal image of human lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD3 (purple) in Cycle 1 and DC-SIGN (cyan) in Cycle 9. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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330111 25 tests $124.00
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330112 100 tests $265.00
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Description

CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a criti­cal role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human DC-SIGN
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1 and spatial biology (IBEX)2,3.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. (IHC-F)
  2. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  3. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Surdziel E, et al. 2017. PLoS One.. 10.1371/journal.pone.0183679. PubMed
  2. Pagan JD, et al. 2018. Cell. 172:564. PubMed
  3. Prasad A, et al. 2017. Sci Rep. 7:40648. PubMed
  4. Olagnier D, et al. 2014. PLoS Pathog. 10:1004566. PubMed
RRID
AB_1186092 (BioLegend Cat. No. 330111)
AB_1186092 (BioLegend Cat. No. 330112)

Antigen Details

Distribution

Dendritic cells

Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.

Gene ID
30835 View all products for this Gene ID
UniProt
View information about CD209 on UniProt.org
Go To Top Version: 4    Revision Date: 04/18/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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