- Clone
- W18340A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ig delta chain C region
- Isotype
- Rat IgG2a, κ
-
IHC staining of anti-human IgD (clone W18340A) Alexa Fluor® 647 on formalin-fixed paraffin-embedded human tonsil tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human IgD (clone W18340A) Alexa Fluor® 647 (red) and anti-human CD45RA (clone HI100) Alexa Fluor® 594 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 324503 | 25 µg | $110.00 | |||
| 324504 | 100 µg | $275.00 | |||
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length human IgD recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- RRID
-
AB_3097334 (BioLegend Cat. No. 324503)
AB_3097334 (BioLegend Cat. No. 324504)
Antigen Details
- Structure
- Exists in a transmembranal and a soluble form
- Distribution
-
Naïve B cells
- Function
- Antigen binding, B cell activation
- Interaction
- The CD79a/CD79b heterodimer
- Molecular Family
- Immune Checkpoint Receptors, Innate Immune Signaling
- Antigen References
-
- Geisberger R, et al. 2006. Immunology 118:429-37.
- Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
- Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
- Gene ID
- 3495 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Other Formats
View All IgD Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IgD | W18340A | IHC-P,SB |
| Alexa Fluor® 647 anti-human IgD | W18340A | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IgD
Human paraffin-embedded tonsil tissue slices were prepared w... 
SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Alexa Fluor® 647 anti-human IgD
IHC staining of anti-human IgD (clone W18340A) Alexa Fluor® ...