- Clone
- W18340A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ig delta chain C region
- Isotype
- Rat IgG2a, κ
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Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, tissue was incubated with goat anti-rat IgG (Poly4054) Alexa Fluor® 594 antibody (red) and anti-human CD19 (clone A17136C) Alexa Fluor® 647 antibody (green) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with software. -
SeqIF™ (sequential immunofluorescence) staining on COMET™ of Purified anti-IgD (clone W18340A, yellow) on formalin-fixed paraffin-embedded human tonsil tissue at 5 µg/mL. Alexa Fluor™ Plus 555 Goat anti-Rat IgG antibody (Lunaphore, Cat. No. DR555RT) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 324502 | 100 µg | $54.00 | |||
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length human IgD recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
SB - Community verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
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For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.
- RRID
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AB_2888899 (BioLegend Cat. No. 324502)
Antigen Details
- Structure
- Exists in a transmembranal and a soluble form
- Distribution
-
Naïve B cells
- Function
- Antigen binding, B cell activation
- Interaction
- The CD79a/CD79b heterodimer
- Molecular Family
- Immune Checkpoint Receptors, Innate Immune Signaling
- Antigen References
-
- Geisberger R, et al. 2006. Immunology 118:429-37.
- Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
- Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
- Gene ID
- 3495 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Other Formats
View All IgD Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IgD | W18340A | IHC-P,SB |
| Alexa Fluor® 647 anti-human IgD | W18340A | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IgD
Human paraffin-embedded tonsil tissue slices were prepared w... 
SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Alexa Fluor® 647 anti-human IgD
IHC staining of anti-human IgD (clone W18340A) Alexa Fluor® ...