Alexa Fluor® 647 anti-mouse CD172a (SIRPα) Antibody

Pricing & Availability
Clone
P84 (See other available formats)
Regulatory Status
RUO
Other Names
SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
Isotype
Rat IgG1, κ
P84_A647_CD172a_Antibody_1_010917
C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD172a (clone P84) Alexa Fluor® 647 (top) or rat IgG1, κ Alexa Fluor® 647 isotype control (bottom). Data shown was gated on myeloid cell populatin.
  • P84_A647_CD172a_Antibody_1_010917
    C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD172a (clone P84) Alexa Fluor® 647 (top) or rat IgG1, κ Alexa Fluor® 647 isotype control (bottom). Data shown was gated on myeloid cell populatin.
  • P84_A647_CD172a_Antibody_2_010917
  • P84_A647_CD172a_Antibody_3_091721
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD200 (OX2) Antibody (clone OX-90) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD172a (SIRPα) Antibody (clone P84) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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Cat # Size Price Quantity Check Availability
144027 25 µg $136.00
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144028 100 µg $347.00
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Description

CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse brain membrane protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
  2. Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
  3. Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
  4. Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  2. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  3. Giampazolias E, et al. 2021. Cell. . PubMed
  4. Tang R, et al. 2020. Elife. 9:00. PubMed
  5. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
RRID
AB_2721300 (BioLegend Cat. No. 144027)
AB_2721300 (BioLegend Cat. No. 144028)

Antigen Details

Structure
Type I transmembrane protein
Distribution

Monocytes, macrophages, myeloid cells, neuronal tissue

Function
Negative regulation of several biological processes
Interaction
PTPN6, PTPN11
Ligand/Receptor
CD47, SP-A, SP-D
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References

1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.

Gene ID
19261 View all products for this Gene ID
UniProt
View information about CD172alpha on UniProt.org
Go To Top Version: 2    Revision Date: 09/17/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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