Alexa Fluor® 647 anti-mouse CD3ε Antibody

Pricing & Availability
Clone
KT3.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
CD3e, T3e
Isotype
Rat IgG2a, κ
KT3-1-1_A647_CD3e_Antibody_091218
C57BL/6 mouse splenocytes were stained with FITC anti-mouse CD19 and with Alexa Fluor® 647 anti-mouse CD3ε (clone KT3.1.1, left) or rat IgG2a, κ isotype control (right).
  • KT3-1-1_A647_CD3e_Antibody_091218
    C57BL/6 mouse splenocytes were stained with FITC anti-mouse CD19 and with Alexa Fluor® 647 anti-mouse CD3ε (clone KT3.1.1, left) or rat IgG2a, κ isotype control (right).
  • KT3-1-1_A647_CD3e_Antibody_2_100119
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD3ε (clone KT3.1.1) Alexa Fluor® 647 (red), anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
155609 25 µg $101.00
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155610 100 µg $229.00
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CBAT6 Thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Tomonari K. 1988. Immunogenetics. 28:455. (FC)
Product Citations
  1. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  2. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
RRID
AB_2750435 (BioLegend Cat. No. 155609)
AB_2750435 (BioLegend Cat. No. 155610)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation
Ligand/Receptor
TCR
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References
  1. Preston S, et al. 1997. Eur. J. Immunol. 27:1911.
  2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
  3. Weiss A, Littman DR. 1994. Cell. 76:263.
Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 2    Revision Date: 10/02/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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