Alexa Fluor® 647 anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
SMI 99 (See other available formats)
Regulatory Status
RUO
Other Names
MBP, Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG2b, κ
SMI-99_A647_MBP_Antibody_1_070919
IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein (clone SMI 99) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI-99_A647_MBP_Antibody_1_070919
    IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein (clone SMI 99) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI-99_A647_MBP_Antibody_2_070919
    IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein (clone SMI 99) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI-99_A647_MBP_Antibody_3_070919
    IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein (clone SMI 99) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
808407 25 µg $159.00
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808408 100 µg $323.00
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the CNS. MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of the classic human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. This antibody detects developing and adult myelin, developing oligodedrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections. The combination of this antibody with clone SMI-91 and/or SMI-94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Additional Product Notes

Myelin basic protein exists in multiple isoforms. By WB, clone SMI 99 detects mainly the 21 kD and 28 kD isoforms in the murine brain tissue, and the 21 kD isoform in the human brain tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Fyffe-Maricich SL, et al. 2011. J. Neurosci. 31(3):843-50. (IHC, WB) PubMed
  2. Mendz GL, et al. 1985. Biochem. 228(1):61-8.
  3. Kerlero De Rosbo N, et al. 1984. Neurochem. Res. 9(10):1359-69.
  4. Carnegie PR, et al. 1983. J. Neuroimmunol. 5(2):125-34.
  5. Dowse CA, et al. 1983. J. Neuroimmunol. 5(2):135-44.
  6. Del Valle L,et al. Cancer Biol Ther. 9:286. (IHC) PubMed
  7. Kiryu-Seo S, et al. 2010. J. Neurosci. 30(19):6658-66. (ICC) PubMed
  8. Samanta J, et al. 2010. Stroke. 41(2):357-62. (IHC) PubMed
  9. Werner HB, et al. 2007. J. Neurosci. 27(29):7717-30. (WB, ICC) PubMed
RRID
AB_2814551 (BioLegend Cat. No. 808407)
AB_2814551 (BioLegend Cat. No. 808408)

Antigen Details

Cell Type
Oligodendrocytes
Biology Area
Cell Biology, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules
Gene ID
4155 View all products for this Gene ID 17196 View all products for this Gene ID 24547 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org
Go To Top Version: 1    Revision Date: 07/09/2019

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