- Clone
- W15102A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NTAL, LAT2, WBS15, LAB
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes were surface stained with CD19 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with W15102A Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom). -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 699005 | 25 tests | $159.00 | |||
| 699006 | 100 tests | $364.00 | |||
Non-T cell activation linker (NTAL) functions as transmembrane adaptor scaffold that links engagement of antigen receptor binding to downstream effector molecules, playing an essential role in maturation, activation, and differentiation in various cell types. It is important for BCR (B-cell antigen receptor) and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-signaling in B-cells and myeloid cells, respectively, and plays a critical role in FCER1 (high affinity immunoglobulin epsilon receptor) signaling in mast cells. NTAL is encoded by the LAT2 gene, which is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human NTAL recombinant protein (27-243 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This antibody does not cross-react with mouse NTAL (in-house tested). Daudi cells were selected for antibody testing due to the cell line’s low expression level of the mRNA encoding NTAL (source: Human Protein Atlas).
<p><strong>NOTE</strong>: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. <a href="http://www.biolegend.com/transcription-factor-f-p-buffer-set-10859.html">424401</a>) offers improved staining and is highly recommended.</p>
- RRID
-
AB_2721434 (BioLegend Cat. No. 699005)
AB_2721434 (BioLegend Cat. No. 699006)
Antigen Details
- Structure
- 243 amino acids with a predicted molecular weight of 26.5 kD, palmitoylation moiety permits localization to lipid rafts or tetraspanin-enriched microdomains.
- Distribution
-
Plasma membrane
- Function
- NTAL is extensively phosphorylated on multiple tyrosine residues following activation, enabling the protein to serve as a scaffold for multiple signaling complexes in various cell contexts. May also be post-translationally regulated by Caspase-3-mediated proteolytic cleavage in human B cells and monocytes.
- Interaction
- Phospholipase C gamma 1 and 2. When phosphorylated, interacts with GRB2. May also interact with SOS1, GAB1, and CBL.
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
-
1. Arbulo-Echevarria MM, et al. 2016. J. Leukoc Biol. 100:351.
2. Stepanek O, et al. 2014. Cell Signal. 5:895.
3. Orr Sj, et al. 2013. Plos Pathog. 9: e1003357.
4. Halova I, et al. 2013. J. Biol. Chem. 288:9801.
5. Duque-Alfonso J, et al. 2011. Oncogene. 30:3062 - Gene ID
- 7462 View all products for this Gene ID
- UniProt
- View information about NTAL on UniProt.org
Other Formats
View All NTAL Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NTAL (LAT2) | W15102A | WB,FC |
| Alexa Fluor® 647 anti-NTAL (LAT2) | W15102A | ICFC |
| TotalSeq™-B1124 anti-NTAL (LAT2) | W15102A | ICPG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-NTAL (LAT2)
Total cell lysates from Raw264.7 (negative control, Lane 1),... 
Human peripheral blood mononuclear cells were treated with T... 
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Alexa Fluor® 647 anti-NTAL (LAT2)
Human peripheral blood lymphocytes were surface stained with... 
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TotalSeq™-B1124 anti-NTAL (LAT2)