- Clone
- W15102A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NTAL, LAT2, WBS15, LAB
- Isotype
- Mouse IgG1, κ
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Total cell lysates from Raw264.7 (negative control, Lane 1), HEK293T (negative control, Lane 2), Daudi (Lane 3) , and THP-1 (Lane 4) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL (1:500) purified anti-NTAL (LAT2) clone 15102A (left) antibody and a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (cat #405301) for clone W15102A, and HRP donkey anti-rabbit IgG (cat #406401) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat no. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: Molecular weight ladder. -
Human peripheral blood mononuclear cells were treated with True-Nuclear™ Transcription Factor Buffer Set then stained with purified anti-NTAL (LAT2) antibody (clone W15102A (filled histogram) or purified mouse IgG1, κ isotype control (clone MOPC-21) (dashed histogram) followed by PE goat anti-mouse IgG antibody (clone Poly4053). Lyphocyte population gating (top), monocyte population gating (bottom). -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 699001 | 25 µg | $101.00 | |||
| 699002 | 100 µg | $253.00 | |||
Non-T cell activation linker (NTAL) functions as transmembrane adaptor scaffold that links engagement of antigen receptor binding to downstream effector molecules, playing an essential role in maturation, activation, and differentiation in various cell types. It is important for BCR (B-cell antigen receptor) and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-signaling in B-cells and myeloid cells, respectively, and plays a critical role in FCER1 (high affinity immunoglobulin epsilon receptor) signaling in mast cells. NTAL is encoded by the LAT2 gene, which is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human NTAL recombinant protein (27-243 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
FC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500-5000). For flow cytometric staining, the suggested use of this reagent is ≤0.00375 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody does not cross-react with mouse NTAL (in-house tested). Daudi cells were selected for antibody testing due to the cell line’s low expression level of the mRNA encoding NTAL (source: Human Protein Atlas).
- RRID
-
AB_2715811 (BioLegend Cat. No. 699001)
AB_2715811 (BioLegend Cat. No. 699002)
Antigen Details
- Structure
- 243 amino acids with a predicted molecular weight of 26.5 kD, palmitoylation moiety permits localization to lipid rafts or tetraspanin-enriched microdomains.
- Distribution
-
Plasma membrane
- Function
- NTAL is extensively phosphorylated on multiple tyrosine residues following activation, enabling the protein to serve as a scaffold for multiple signaling complexes in various cell contexts. May also be post-translationally regulated by Caspase-3-mediated proteolytic cleavage in human B cells and monocytes.
- Interaction
- Phospholipase C gamma 1 and 2. When phosphorylated, interacts with GRB2. May also interact with SOS1, GAB1, and CBL.
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
-
1. Arbulo-Echevarria MM, et al. 2016. J. Leukoc Biol. 100:351.
2. Stepanek O, et al. 2014. Cell Signal. 5:895.
3. Orr Sj, et al. 2013. Plos Pathog. 9: e1003357.
4. Halova I, et al. 2013. J. Biol. Chem. 288:9801.
5. Duque-Alfonso J, et al. 2011. Oncogene. 30:3062 - Gene ID
- 7462 View all products for this Gene ID
- UniProt
- View information about NTAL on UniProt.org
Other Formats
View All NTAL Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NTAL (LAT2) | W15102A | WB,FC |
| Alexa Fluor® 647 anti-NTAL (LAT2) | W15102A | ICFC |
| TotalSeq™-B1124 anti-NTAL (LAT2) | W15102A | ICPG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-NTAL (LAT2)
Total cell lysates from Raw264.7 (negative control, Lane 1),... 
Human peripheral blood mononuclear cells were treated with T... 
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Alexa Fluor® 647 anti-NTAL (LAT2)
Human peripheral blood lymphocytes were surface stained with... 
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TotalSeq™-B1124 anti-NTAL (LAT2)