Alexa Fluor® 700 anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
OKT3_AF700_CD3_Antibody_FC_032014.jpg
Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) Alexa Fluor® 700 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
  • OKT3_AF700_CD3_Antibody_FC_032014.jpg
    Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) Alexa Fluor® 700 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
317339 25 tests $116.00
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317340 100 tests $248.00
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  2. Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed
  3. Aarsund M, et al. 2022. Cancer Immunol Immunother. 71:2227. PubMed
  4. Tarke A, et al. 2021. Cell Reports Medicine. 2(2):100204. PubMed
  5. Muyayalo KP, et al. 2023. Am J Reprod Immunol. 89:e13591. PubMed
  6. Fendler A, et al. 2022. Cell Rep Med. 3:100781. PubMed
  7. Ke F, et al. 2023. Elife. 12:. PubMed
  8. Groß R, et al. 2022. EBioMedicine. 75:103761. PubMed
  9. Carbonneau S, et al. 2020. Cell Chemical Biology. 28(6):802-812.e6. PubMed
  10. Li N, et al. 2021. STAR Protoc. 2:100942. PubMed
  11. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  12. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  13. Grifoni A, et al. 2020. J Virol. 94:00:00. PubMed
  14. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  15. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  16. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  17. Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed
  18. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
  19. Voic H, et al. 2020. J Virol. 94: . PubMed
  20. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
  21. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  22. Dettling S, et al. 2018. Clin Cancer Res. 24(12). PubMed
  23. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
RRID
AB_2563407 (BioLegend Cat. No. 317339)
AB_2563407 (BioLegend Cat. No. 317340)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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