Alexa Fluor® 700 anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
1_RPA-T4_Alx700_080408.jpg
Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 700
  • 1_RPA-T4_Alx700_080408.jpg
    Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 700
  • 2_67_Human_Metastatic_Lymph_Node_CD4_EpCAM
    Confocal image of human metastatic lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD4 (blue) in Cycle 1 and EpCAM (red) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 700 spectral data
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300526 100 µg $268.00
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  2. Saavedra D, et al. 2022. Immunol Lett. :1. PubMed
  3. Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed
  4. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  5. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  6. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  7. Hubbard N, et al. 2016. Blood. 127: 2513 - 2522. PubMed
  8. Taura M, et al. 2019. Proc Natl Acad Sci U S A. 116:2282. PubMed
  9. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  10. Elmentaite R, et al. 2021. Nature. 597:250. PubMed
  11. Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
  12. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  13. Kilgour MK, et al. 2022. Nat Protoc. 17:2668. PubMed
  14. Presicce P, et al. 2011. PLoS One. 6:e28118. PubMed
  15. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  16. Wild K, et al. 2021. Nat Commun. 12:6720. PubMed
  17. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  18. Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
  19. Newton HS, et al. 2021. Mol Ther Methods Clin Dev. 21:133. PubMed
  20. Platten M, et al. 2021. Nature. 592:463. PubMed
  21. Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
  22. Simpfendorfer K, et al. 2012. Hum Mol Genet. 21:3918. PubMed
  23. Oberhardt V, et al. 2021. Nature. 597:268. PubMed
  24. Muller YD, et al. 2021. Front Immunol. 12:639818. PubMed
  25. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  26. Carnevale G,et al. 2017. Sci Rep.. 10.1038/s41598-017-14838-3. PubMed
  27. Dupraz L, et al. 2021. Cell Reports. 36(1):109332. PubMed
  28. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  29. Presicce P, et al. 2012. PLoS One. 7:e42802. PubMed
  30. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  31. Toor SM, et al. 2018. Clin Exp Immunol. 191:189. PubMed
RRID
AB_493743 (BioLegend Cat. No. 300526)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD4 RPA-T4 FC
Biotin anti-human CD4 RPA-T4 FC
FITC anti-human CD4 RPA-T4 FC,SB
PE anti-human CD4 RPA-T4 FC
PE/Cyanine5 anti-human CD4 RPA-T4 FC
PE/Cyanine7 anti-human CD4 RPA-T4 FC
Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
APC/Cyanine7 anti-human CD4 RPA-T4 FC
Alexa Fluor® 488 anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 647 anti-human CD4 RPA-T4 FC,SB
Pacific Blue™ anti-human CD4 RPA-T4 FC
Brilliant Violet 421™ anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 700 anti-human CD4 RPA-T4 FC,SB
PerCP anti-human CD4 RPA-T4 FC
PerCP/Cyanine5.5 anti-human CD4 RPA-T4 FC
Brilliant Violet 570™ anti-human CD4 RPA-T4 FC
Brilliant Violet 650™ anti-human CD4 RPA-T4 FC
Purified anti-human CD4 (Maxpar® Ready) RPA-T4 FC,CyTOF®
Alexa Fluor® 594 anti-human CD4 RPA-T4 FC,ICC
Brilliant Violet 510™ anti-human CD4 RPA-T4 FC
PE/Dazzle™ 594 anti-human CD4 RPA-T4 FC
Brilliant Violet 785™ anti-human CD4 RPA-T4 FC
Brilliant Violet 605™ anti-human CD4 RPA-T4 FC
Brilliant Violet 711™ anti-human CD4 RPA-T4 FC
APC/Fire™ 750 anti-human CD4 RPA-T4 FC
CD4 Fluorophore Sampler Kit RPA-T4 FC
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC RPA-T4 FC
TotalSeq™-A0072 anti-human CD4 RPA-T4 PG
TotalSeq™-B0072 anti-human CD4 RPA-T4 PG
TotalSeq™-C0072 anti-human CD4 RPA-T4 PG
Ultra-LEAF™ Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
TotalSeq™-D0072 anti-human CD4 RPA-T4 PG
Go To Top Version: 4    Revision Date: 04/26/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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