PE/Cyanine7 anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
RPA-T4_PECy7_060507
Human peripheral blood lymphocytes stained with RPA-T4 PE/Cyanine7
  • RPA-T4_PECy7_060507
    Human peripheral blood lymphocytes stained with RPA-T4 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
300511 25 tests $143.00
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300512 100 tests $292.00
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  2. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  3. Jaiswal A, et al. 2022. Cancer Cell. 40:524. PubMed
  4. Ratnapriya S, et al. 2021. Cell Reports. 36(9):109622. PubMed
  5. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  6. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
  7. Rose JR, et al. 2023. Commun Biol. 6:363. PubMed
  8. Saotome K, et al. 2023. Nat Commun. 14:2401. PubMed
  9. Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed
  10. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  11. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  12. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  13. Malinarich F, et al. 2015. J Immunol. 194:5174. PubMed
  14. Arra A, et al. 2021. Oncoimmunology. 10:1938475. PubMed
  15. Chung B, et al. 2012. PLoS One. 7:e42011. PubMed
  16. Wu VH, et al. 2020. JCI Insight. 5:00. PubMed
  17. Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
  18. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  19. Yu N, et al. 2018. Genes Immun. 10.1038/s41435-017-0007-7. PubMed
  20. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  21. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  22. Romero-Olmedo AJ, et al. 2022. Nat Microbiol. 7:195. PubMed
  23. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  24. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  25. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  26. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  27. Albanese M, et al. 2022. Nat Methods. 19:81. PubMed
  28. Park JS, et al. 2022. Arthritis Res Ther. 24:254. PubMed
  29. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  30. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  31. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  32. Chung B, et al. 2015. PLoS One. 10: 0145258. PubMed
  33. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  34. Brunsvig PF, et al. 2020. Front Immunol. 11:572172. PubMed
  35. Masuda N, et al. 2021. BMC Cancer. 21:1269. PubMed
  36. Terzioglu G,et al. 2017. Mech Ageing Dev. . 10.1016/j.mad.2017.09.003. PubMed
  37. Min HK, et al. 2021. Immune Netw. 21:e43. PubMed
  38. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  39. Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
  40. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  41. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  42. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  43. Hassounah NB, et al. 2019. Cancer Immunol Immunother. 68:407. PubMed
RRID
AB_314079 (BioLegend Cat. No. 300511)
AB_314079 (BioLegend Cat. No. 300512)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD4 RPA-T4 FC
Biotin anti-human CD4 RPA-T4 FC
FITC anti-human CD4 RPA-T4 FC,SB
PE anti-human CD4 RPA-T4 FC
PE/Cyanine5 anti-human CD4 RPA-T4 FC
PE/Cyanine7 anti-human CD4 RPA-T4 FC
Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
APC/Cyanine7 anti-human CD4 RPA-T4 FC
Alexa Fluor® 488 anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 647 anti-human CD4 RPA-T4 FC,SB
Pacific Blue™ anti-human CD4 RPA-T4 FC
Brilliant Violet 421™ anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 700 anti-human CD4 RPA-T4 FC,SB
PerCP anti-human CD4 RPA-T4 FC
PerCP/Cyanine5.5 anti-human CD4 RPA-T4 FC
Brilliant Violet 570™ anti-human CD4 RPA-T4 FC
Brilliant Violet 650™ anti-human CD4 RPA-T4 FC
Purified anti-human CD4 (Maxpar® Ready) RPA-T4 FC,CyTOF®
Alexa Fluor® 594 anti-human CD4 RPA-T4 FC,ICC
Brilliant Violet 510™ anti-human CD4 RPA-T4 FC
PE/Dazzle™ 594 anti-human CD4 RPA-T4 FC
Brilliant Violet 785™ anti-human CD4 RPA-T4 FC
Brilliant Violet 605™ anti-human CD4 RPA-T4 FC
Brilliant Violet 711™ anti-human CD4 RPA-T4 FC
APC/Fire™ 750 anti-human CD4 RPA-T4 FC
CD4 Fluorophore Sampler Kit RPA-T4 FC
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC RPA-T4 FC
TotalSeq™-A0072 anti-human CD4 RPA-T4 PG
TotalSeq™-B0072 anti-human CD4 RPA-T4 PG
TotalSeq™-C0072 anti-human CD4 RPA-T4 PG
Ultra-LEAF™ Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
TotalSeq™-D0072 anti-human CD4 RPA-T4 PG
Go To Top Version: 2    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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