Alexa Fluor® 700 anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
OKT4_Alx700_072408.jpg
Human peripheral blood lymphocytes stained with OKT4 Alexa Fluor® 700
  • OKT4_Alx700_072408.jpg
    Human peripheral blood lymphocytes stained with OKT4 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
317425 25 µg $124.00
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317426 100 µg $265.00
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Paniskaki K, et al. 2022. Transplant Proc. 54:1455. PubMed
  2. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  3. Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed
  4. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  5. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  6. Lovelace SE, et al. 2022. iScience. 25:105067. PubMed
  7. Gordon E, et al. 2016. Proc Natl Acad Sci U S A. 113: 8765 - 8770. PubMed
  8. Cerosaletti K, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1700172. PubMed
  9. Naaber P, et al. 2022. Cell Rep Med. 3:100716. PubMed
  10. Ott PA, et al. 2017. Nature. 547:217. PubMed
  11. Asokan M, et al. 2020. Proc Natl Acad Sci U S A. 117:18754. PubMed
  12. Aschauer C, et al. 2021. Front Immunol. 12:750005. PubMed
  13. Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed
  14. Frencher J, et al. 2014. J Leukoc Biol. 96:957. PubMed
  15. Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed
  16. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  17. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  18. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  19. Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed
RRID
AB_571943 (BioLegend Cat. No. 317425)
AB_571943 (BioLegend Cat. No. 317426)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
Go To Top Version: 5    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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