Alexa Fluor 700 anti-human IgM Antibody anti-IgM

Pricing & Availability

Clone: MHM-88 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ

MHM-88_A700_IgM_Antibody_1_120115 — Overnight cultured human peripheral blood mononuclear cells were stained with CD19 PE and IgM (clone MHM-88) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom). Data shown was gated on lymphocyte population.

Compare all formats See Alexa Fluor® 700 spectral data

Cat #

Size

Price

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314537

25 tests

$165.00

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314538

100 tests

$347.00

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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human Ig cocktail

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References (PubMed link indicates BioLegend citation)

Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed

Product Citations

Parry H, et al. 2022. Cell Rep Med. 3:100739. PubMed
Li L, et al. 2023. PLoS Pathog. e1011856:19. PubMed
Wang Z, et al. 2022. J Exp Med. 219: . PubMed
Xu C, et al. 2022. iScience. 25:105123. PubMed
Schaefer-Babajew D, et al. 2023. Nature. 613:735. PubMed
Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
Cho A, et al. 2021. Nature. Online ahead of print. PubMed
Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
Wang Z, et al. 2021. Nature. 595:426. PubMed
Cho A, et al. 2022. J Exp Med. 219:. PubMed

RRID

AB_2566615 (BioLegend Cat. No. 314537)
AB_2566615 (BioLegend Cat. No. 314538)

Antigen Details

Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507 View all products for this Gene ID
UniProt: View information about IgM on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Go To Top Version: 1 Revision Date: 12/07/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.