FITC anti-human IgM Antibody

Pricing & Availability
Clone
MHM-88 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG1, κ
MHM-88_FITC_021009.jpg
Human peripheral blood lymphocytes stained with MHM-88 FITC
  • MHM-88_FITC_021009.jpg
    Human peripheral blood lymphocytes stained with MHM-88 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
314506 100 tests $188.00
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  2. Watanabe A, et al. 2019. Cell. 177:1124. PubMed
  3. Nagashima K, et al. 2022. J Immunol. 209:5. PubMed
  4. Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed
  5. Zhao Y, et al. 2022. Front Immunol. 13:1041176. PubMed
  6. Braham MVJ, et al. 2023. iScience. 26:105741. PubMed
  7. Sungalee S, et al. 2021. Nat Genet. 53:650. PubMed
  8. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  9. Muir L, et al. 2018. Wellcome Open Res. 2:97. PubMed
  10. Fenwick C, et al. 2022. Nat Microbiol. 7:1376. PubMed
  11. Lindner JM, et al. 2019. Immunity. 50:668. PubMed
  12. Zost SJ, et al. 2020. Nat Med. 26:1422. PubMed
  13. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  14. Zost SJ, et al. 2020. bioRxiv. . PubMed
  15. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  16. Ros F, et al. 2020. MAbs. 12:1846900. PubMed
  17. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  18. Lisboa F, et al. 2011. J Biol Chem. 286:7577. PubMed
  19. Nicolò A, et al. 2022. Front Immunol. 13:1016263. PubMed
  20. Benhammou JN, et al. 2020. Dig Dis Sci. 66:2394. PubMed
  21. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  22. Fenwick C, et al. 2021. Cell Rep. 37:109814. PubMed
  23. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  24. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  25. Ju L,et al. 2017. Sci Rep.. 10.1038/s41598-017-13793-3. PubMed
  26. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  27. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  28. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
RRID
AB_493009 (BioLegend Cat. No. 314506)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Other Formats

View All IgM Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IgM MHM-88 FC,ELISA
PE anti-human IgM MHM-88 FC
Biotin anti-human IgM MHM-88 FC,ELISA
FITC anti-human IgM MHM-88 FC
APC anti-human IgM MHM-88 FC
PerCP/Cyanine5.5 anti-human IgM MHM-88 FC
Pacific Blue™ anti-human IgM MHM-88 FC
Brilliant Violet 421™ anti-human IgM MHM-88 FC
APC/Cyanine7 anti-human IgM MHM-88 FC
Brilliant Violet 570™ anti-human IgM MHM-88 FC
Brilliant Violet 510™ anti-human IgM MHM-88 FC
Brilliant Violet 605™ anti-human IgM MHM-88 FC
Brilliant Violet 650™ anti-human IgM MHM-88 FC
Purified anti-human IgM (Maxpar® Ready) MHM-88 FC,CyTOF®
PE/Dazzle™ 594 anti-human IgM MHM-88 FC
PE/Cyanine7 anti-human IgM MHM-88 FC
Alexa Fluor® 488 anti-human IgM MHM-88 FC
Alexa Fluor® 647 anti-human IgM MHM-88 FC
Alexa Fluor® 700 anti-human IgM MHM-88 FC
Brilliant Violet 711™ anti-human IgM MHM-88 FC
TotalSeq™-A0136 anti-human IgM MHM-88 PG
TotalSeq™-C0136 anti-human IgM MHM-88 PG
Brilliant Violet 785™ anti-human IgM MHM-88 FC
APC/Fire™ 750 anti-human IgM MHM-88 FC
TotalSeq™-B0136 anti-human IgM MHM-88 PG
Spark Violet™ 423 anti-human IgM Antibody MHM-88 FC
TotalSeq™-D0136 anti-human IgM MHM-88 PG
Spark Blue™ 550 anti-human IgM MHM-88 FC
Spark Blue™ 515 anti-human IgM MHM-88 FC
PE/Fire™ 700 anti-human IgM MHM-88 FC
Spark YG™ 593 anti-human IgM MHM-88 FC
PE/Fire™ 640 anti-human IgM MHM-88 FC
Spark Red™ 718 anti-human IgM (Flexi-Fluor™) MHM-88 FC
APC anti-human IgM MHM-88 FC
PE anti-human IgM MHM-88 FC
Brilliant Violet 750™ anti-human IgM MHM-88 FC
Go To Top Version: 3    Revision Date: 10/10/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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