APC anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody

Pricing & Availability
Clone
6B8B69 (See other available formats)
Regulatory Status
RUO
Other Names
P44/42 MAP (mitogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype
Mouse IgG2a, κ
6B8B69_APC_ERK1salsh2_Antibody_032218
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A, Cat No. 423301) for 15 minutes, then fixed with Fixation Buffer (Cat No. 420801), permeabilized with cold True-Phos™ Perm Buffer (Cat No. 425401), and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (clone 6B8B69) APC .
  • 6B8B69_APC_ERK1salsh2_Antibody_032218
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A, Cat No. 423301) for 15 minutes, then fixed with Fixation Buffer (Cat No. 420801), permeabilized with cold True-Phos™ Perm Buffer (Cat No. 425401), and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (clone 6B8B69) APC .
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369521 25 tests $142.00
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369522 100 tests $350.00
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Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes.

Product Citations
  1. Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed
RRID
AB_2728374 (BioLegend Cat. No. 369521)
AB_2728374 (BioLegend Cat. No. 369522)

Antigen Details

Structure
ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution

Cytoplasm and the nucleus.

Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.
Interaction
ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.

Gene ID
5594 View all products for this Gene ID
UniProt
View information about ERK1/2 on UniProt.org
Go To Top Version: 1    Revision Date: 03/23/2018

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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