Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody

Pricing & Availability
Clone
6B8B69 (See other available formats)
Regulatory Status
RUO
Other Names
P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype
Mouse IgG2a, κ
6B8B69_PURE_ERK1slash2_Antibody_WB_010817
Total cell lysates (15 µg protein) from non-treated (lane 1) and PMA treated (100 ng/mL treatment for 5 minutes, lane 2) Jurkat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL), 1:2000 diluted (0.25 µg/mL) and 1:5000 diluted (0.1 µg/mL) anti-ERK1/2 Phospho (Thr202/Tyr204) antibody (clone 6B8B69) antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: protein marker.
  • 6B8B69_PURE_ERK1slash2_Antibody_WB_010817
    Total cell lysates (15 µg protein) from non-treated (lane 1) and PMA treated (100 ng/mL treatment for 5 minutes, lane 2) Jurkat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL), 1:2000 diluted (0.25 µg/mL) and 1:5000 diluted (0.1 µg/mL) anti-ERK1/2 Phospho (Thr202/Tyr204) antibody (clone 6B8B69) antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: protein marker.
  • 6B8B69_PURE_Phospho_051722
    HeLa cells were treated with (+) or without (-) 100 ng/mL PFA for 5 minutes, fixed with ice-cold MeOH for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with either purified mouse IgG1, κ isotype control antibody (Cat. No. 401402) (panel A) or purified anti-ERK1/2 Phospho (Thr202/Tyr204) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.5 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scalebar: 50 µm
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Cat # Size Price Quantity Check Availability
369501 25 µg $112.00
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369502 100 µg $265.00
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Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1: 5000 diluted (0.1-1.0 µg/ml). For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes.

Product Citations
  1. Recabal A, et al. 2020. Journal of Neurochemistry. 156(2):182-199. PubMed
RRID
AB_2721735 (BioLegend Cat. No. 369501)
AB_2721735 (BioLegend Cat. No. 369502)

Antigen Details

Structure
ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution

Cytoplasm and the nucleus.

Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.
Interaction
ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 
5. Torii S, et al. 2004. J. Biochem. 136:557. 
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.

Gene ID
5594 View all products for this Gene ID
UniProt
View information about ERK1/2 on UniProt.org
Go To Top Version: 1    Revision Date: 05/17/2022

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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