APC anti-human CD34 Antibody

Pricing & Availability
Clone
581 (See other available formats)
Regulatory Status
RUO
Workshop
V MA27
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
A_581_APC_091009
Human peripheral blood leukocytes stained with 581 APC/CD45 FITC/CD14 PE and 7-AAD then analyzed via gating on total live and CD14 negative lymphocyte population
  • A_581_APC_091009
    Human peripheral blood leukocytes stained with 581 APC/CD45 FITC/CD14 PE and 7-AAD then analyzed via gating on total live and CD14 negative lymphocyte population
  • B_581_APC_091009
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Cat # Size Price Quantity Check Availability
343509 25 tests $113.00
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343510 100 tests $286.00
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Schelker RC, et al. 2018. Hematology. 1.192361111. PubMed
  2. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  3. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  4. Minami H, et al. 2015. PLoS One. 10:128890. PubMed
  5. Sun Z, et al. 2022. Stem Cell Res Ther. 13:297. PubMed
  6. Sharma R, et al. 2021. Nat Commun. 12:472. PubMed
  7. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  8. Kohn L, et al. 2012. Nat Immunol. 13:963. PubMed
  9. Gomez–Ospina N, et al. 2019. Nat Commun. 3.225694444. PubMed
  10. Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed
  11. Blom T, et al. 2021. EJNMMI Res. 11:81. PubMed
  12. Peters S, et al. 2018. Front Neurol. 0.797916667. PubMed
  13. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  14. Aerts-Kaya FSF, et al. 2020. Biol Blood Marrow Transplant. 634:26. PubMed
  15. Okumura T, et al. 2019. Stem Cell Res Ther. 10:185. PubMed
  16. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  17. Grassinger J, et al. 2014. Cytokine. 67:21. PubMed
  18. Scharenberg SG, et al. 2020. Nat Commun. 2.76875. PubMed
RRID
AB_1877153 (BioLegend Cat. No. 343509)
AB_1877153 (BioLegend Cat. No. 343510)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 2    Revision Date: 03/18/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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