APC anti-mouse IL-6 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7-expressed, recombinant mouse IL-6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody can neutralize the bioactivity of natural or recombinant IL-6. Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

ELISA Capture^1-3,11 or ELISPOT Capture^4,5: The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.

Neutralization^1-2,8-10: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. No. 504506).

Additional reported applications (for relevant formats of this clone) include: In Vivo Capture⁷, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated⁶, and acetone-fixed frozen tissue sections^{13, 14}).

Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)

1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA Capture, Neut)
3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA Capture)
4. Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181. (ELISPOT Capture)
5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT Capture)
6. Litton M, et al. 1994. J. Immunol Meth. 175:47. (IHC)
7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28. (In Vivo Capture)
8. Starnes HF Jr, et al. 1990. J. Immunol. 145:4185. (Neut)
9. Riedemann NC, et al. 2003. J. Immunol. 170:503. (Neut)
10. Rochman I, et al. 2005. J. Immunol. 174:4761. (Neut)
11. O'Connell PJ, et al. 2006. Blood 107:1010. (ELISA Capture)
12. Ozeki Y, et al. 2010. Int. Immunol. 22:179. PubMed
13. Galie M, et al. 2005. Carcinogenesis 26:1868 (IHC)
14. Schone MP, et al. 2000. J Immunol 165:6583 (IHC)
15. Keswani T, et al. 2014. Exp Parasitol. 141:82. PubMed
16. Notley CA, et al. 2015. J Immunol. 194:1621. PubMed

Product Citations

1. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
2. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
3. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
4. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
5. Zou L, et al. 2022. Brain Behav Immun. 100:10. PubMed
6. Saito S, et al. 2020. Nutrients. 12:. PubMed
7. Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed
8. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
9. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
10. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
11. Paranjape A, et al. 2016. J Leukoc Biol. 100(6):1395-1404. PubMed
12. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
13. Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
14. Sung PS, et al. 2022. JCI Insight. :. PubMed
15. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
16. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
17. Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
18. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
19. Liu Y, et al. 2022. Exp Anim. :. PubMed
20. Chen KE, et al. 2021. J Immunol. 206:141. PubMed
21. Orozco SL, et al. 2019. Cell Rep. 28:2275. PubMed

RRID

AB_10694868 (BioLegend Cat. No. 504507)
AB_10694868 (BioLegend Cat. No. 504508)

Antigen Details

Structure

Neuropoietic cytokine family; 22-29 kD (Mammalian)

Bioactivity

Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction

Cell Sources

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Cell Targets

T cells, B cells, hepatocytes, cholinergic neurons

Receptors

Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)

Biology Area

Immunology, Innate Immunity

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Regulation

Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β

Gene ID

16193 View all products for this Gene ID

UniProt

View information about IL-6 on UniProt.org

Related Protocols

• Surface and Intracellular Cytokine Staining for Flow Cytometry - Video
• Intracellular Flow Cytometry Staining Protocol

Other Formats