PE anti-mouse IL-6 Antibody

Pricing & Availability
Clone
MP5-20F3 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG1, κ
MP5-20F3_PE_1_120210
Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stimulated with LPS for 6 hours (in the presence of monensin), then surface stained with anti-mouse CD107b/Mac-3 APC. This was followed by fixation and permeabilization and then stained with anti-mouse IL-6 (MP5-20F3) PE (top) or rat IgG1, κ PE isotype control (bottom).
  • MP5-20F3_PE_1_120210
    Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stimulated with LPS for 6 hours (in the presence of monensin), then surface stained with anti-mouse CD107b/Mac-3 APC. This was followed by fixation and permeabilization and then stained with anti-mouse IL-6 (MP5-20F3) PE (top) or rat IgG1, κ PE isotype control (bottom).
  • MP5-20F3_PE_2_120210
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Cat # Size Price Quantity Check Availability
504503 25 µg $96.00
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504504 100 µg $237.00
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Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7-expressed, recombinant mouse IL-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody can neutralize the bioactivity of natural or recombinant IL-6. Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture1-3,11 or ELISPOT Capture4,5: The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.

Neutralization1-2,8-10: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. No. 504506).

Additional reported applications (for relevant formats of this clone) include: In Vivo Capture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).

Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA Capture, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA Capture)
  4. Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181. (ELISPOT Capture)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT Capture)
  6. Litton M, et al. 1994. J. Immunol Meth. 175:47. (IHC)
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28. (In Vivo Capture)
  8. Starnes HF Jr, et al. 1990. J. Immunol. 145:4185. (Neut)
  9. Riedemann NC, et al. 2003. J. Immunol. 170:503. (Neut)
  10. Rochman I, et al. 2005. J. Immunol. 174:4761. (Neut)
  11. O'Connell PJ, et al. 2006. Blood 107:1010. (ELISA Capture)
  12. Ozeki Y, et al. 2010. Int. Immunol. 22:179. PubMed
  13. Galie M, et al. 2005. Carcinogenesis 26:1868 (IHC)
  14. Schone MP, et al. 2000. J Immunol 165:6583 (IHC)
  15. Keswani T, et al. 2014. Exp Parasitol. 141:82. PubMed
  16. Notley CA, et al. 2015. J Immunol. 194:1621. PubMed
Product Citations
  1. Khan NZ, et al. 2021. Brain Behav Immun. 92:165. PubMed
  2. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  3. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  4. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  5. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  6. Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed
  7. Castoldi Â, et al. 2020. Nat Commun. 3.310416667. PubMed
  8. Prystaz K, et al. 2018. Am J Pathol. 188:474. PubMed
  9. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  10. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed
  11. Jin Q, et al. 2022. Drug Des Devel Ther. 16:3297. PubMed
  12. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  13. Yu C, et al. 2015. J Immunol. 195: 3890 - 3900. PubMed
  14. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  15. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  16. Piliponsky AM, et al. 2019. Nat Immunol. 0.922916667. PubMed
  17. Zhu M, et al. 2015. J Immunol. 195: 4492 - 4502. PubMed
  18. Meher A, et al. 2014. Am J Pathol. 184:3130. PubMed
  19. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  20. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  21. Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed
  22. Li C, et al. 2017. Nat Commun. 8:14275. PubMed
  23. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  24. Lyons DO, et al. 2018. PLoS One. 13:e0207704. PubMed
  25. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
RRID
AB_315337 (BioLegend Cat. No. 504503)
AB_315337 (BioLegend Cat. No. 504504)

Antigen Details

Structure
Neuropoietic cytokine family; 22-29 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Regulation
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-6 MP5-20F3 ICFC
Purified anti-mouse IL-6 MP5-20F3 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,IHC-F
APC anti-mouse IL-6 MP5-20F3 ICFC
Purified anti-mouse IL-6 (Maxpar® Ready) MP5-20F3 ELISA Capture,CyTOF®
Ultra-LEAF™ Purified anti-mouse IL-6 MP5-20F3 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,IHC-F
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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