APC/Cyanine7 anti-mouse CD326 (Ep-CAM) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

TE-71 thymic epithelial cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed¹, with or without OCT embedding^2,4, and spatial biology (IBEX)^13,14.

Application References

(PubMed link indicates BioLegend citation)

1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
7. Ishii K, et al. 2012. Development. 139:1734. PubMed
8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Zwarycz B, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:1. PubMed
2. Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed
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5. Lorenzo-Martín LF, et al. 2022. Oncogene. 41:3341. PubMed
6. Krishnamurty AT, et al. 2022. Nature. 611:148. PubMed
7. Setia M, et al. 2023. STAR Protoc. 4:102056. PubMed
8. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
9. Weeden C, et al. 2017. PLoS Biol. 10.1371/journal.pbio.2000731. PubMed
10. Karlsson J, et al. 2021. Adv Funct Mater. 31:. PubMed
11. Ruth JR, et al. 2021. Breast Cancer Res. 23:63. PubMed
12. Dumont-Lagacé M, et al. 2014. J Immunol. 192:2219. PubMed
13. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
14. Zaid A, et al. 2014. Proc Natl Acad Sci U S A. 111:5307. PubMed
15. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
16. Xu Q, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
17. Agarwal S, et al. 2015. Cell Rep. 13: 2147-2158. PubMed
18. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
19. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
20. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
21. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
22. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
23. Kim M, et al. 2015. J Immunol. 194:4748. PubMed
24. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
25. Guo Q, et al. 2022. Nat Commun. 13:3837. PubMed
26. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
27. Morral C, et al. 2020. Cell Stem Cell. 26(6):845-861. PubMed
28. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
29. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
30. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
31. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
32. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
33. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
34. Wells KL, et al. 2020. Elife. 9:. PubMed
35. Mulholland D, et al. 2012. Cancer Res. 72:1878. PubMed
36. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
37. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
38. Manzo N, et al. 2012. Am J Respir Cell Mol Biol. 47:349. PubMed
39. Douchi D, et al. 2022. Cell Mol Gastroenterol Hepatol. 13:1317. PubMed
40. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
41. St-Pierre C, et al. 2015. J Immunol. 195: 498 - 506. PubMed
42. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
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44. Ikonomou L, et al. 2020. Nat Commun. 0.899305556. PubMed
45. Pascual R, et al. 2020. Sci Adv. 6:eaax3868. PubMed
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47. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
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50. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
51. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
52. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
53. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
54. Garibaldi B, et al. 2013. Am J Respir Cell Mol Biol. 48:35. PubMed
55. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
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RRID

AB_1501158 (BioLegend Cat. No. 118217)
AB_1501158 (BioLegend Cat. No. 118218)

Antigen Details

Structure

40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.

Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function

Mediates calcium-independent homophilic cell-cell adhesion.

Interaction

CD326 displays hemophilic binding.

Ligand/Receptor

CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.

Cell Type

Embryonic Stem Cells, Epithelial cells

Biology Area

Immunology, Stem Cells

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID

17075 View all products for this Gene ID

UniProt

View information about CD326 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats