PE/Cyanine5 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
G88_PE-Cyanine5_CD326_Antibody_113022
4T1 (a mouse triple negative breast cancer cell line) cells were stained with anti-mouse CD326 (clone G8.8) PE/Cyanine5 (filled histogram) or rat IgG2a, κ PE/Cyanine5 isotype control (open histogram).
  • G88_PE-Cyanine5_CD326_Antibody_113022
    4T1 (a mouse triple negative breast cancer cell line) cells were stained with anti-mouse CD326 (clone G8.8) PE/Cyanine5 (filled histogram) or rat IgG2a, κ PE/Cyanine5 isotype control (open histogram).
Compare all formats See PE/Cyanine5 spectral data
Cat # Size Price Quantity Check Availability
118248 25 µg $155.00
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118249 100 µg $336.00
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2936690 (BioLegend Cat. No. 118248)
AB_2936690 (BioLegend Cat. No. 118249)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM) G8.8 FC
PE anti-mouse CD326 (Ep-CAM) G8.8 FC
FITC anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 1    Revision Date: 11/30/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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