APC/Cyanine7 anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
H57-597_APCCy7_TCRb_Antibody_FC_080113
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) APC/Cyanine7 (filled histrogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
  • H57-597_APCCy7_TCRb_Antibody_FC_080113
    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) APC/Cyanine7 (filled histrogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
109219 25 µg $94.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109220 100 µg $215.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Dillon T, et al. 2013. Int Immunol. 25:259. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  4. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  5. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  6. Pérez-Torrado V, et al. 2022. Bio Protoc. 12:. PubMed
  7. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  8. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  9. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  10. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  11. Lin CH, et al. 2023. iScience. 26:106904. PubMed
  12. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  13. du Halgouet A, et al. 2023. Immunity. 56:78. PubMed
  14. Xu K, et al. 2021. Immunity. 54(5):976-987.e7. PubMed
  15. Acker A, et al. 2014. PLoS One. 9:110015. PubMed
  16. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  17. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  18. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  19. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  20. Alcaraz-Serna A, et al. 2021. Sci Adv. 7:. PubMed
  21. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  22. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  23. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  24. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  25. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
  26. Harada Y, et al. 2022. iScience. 25:104021. PubMed
  27. Higa T, et al. 2022. Nat Commun. 13:1500. PubMed
  28. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
  29. Broeck T, et al. 2013. J Immunol. 190:1982. PubMed
  30. Hirata Y, et al. 2015. PLoS One. 10: 0141785. PubMed
  31. Devos M, et al. 2020. J Exp Med. 217:00:00. PubMed
  32. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  33. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  34. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  35. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  36. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  37. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  38. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  39. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  40. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  41. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  42. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  43. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  44. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  45. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  46. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  47. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  48. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  49. Jin C, et al. 2019. Cell. 176:998. PubMed
  50. Huang L, et al. 2020. Front Immunol. 10:3103. PubMed
  51. Giampazolias E, et al. 2021. Cell. . PubMed
  52. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  53. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  54. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  55. Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed
  56. Seki N, et al. 2022. iScience. 25:104838. PubMed
  57. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  58. Murata T, et al. 2020. Sci Rep. 10:13560. PubMed
  59. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  60. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  61. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
  62. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  63. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  64. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  65. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  66. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
  67. Wang X, et al. 2022. Elife. 11:. PubMed
  68. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  69. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  70. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  71. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  72. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  73. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
  74. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_893626 (BioLegend Cat. No. 109219)
AB_893626 (BioLegend Cat. No. 109220)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark PLUS UV395™ anti-mouse TCR β chain H57-597 FC
TotalSeq™-D0120 anti-mouse TCR β chain H57-597 PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 3    Revision Date: 02/06/2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account