- Clone
- S19021B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PTPRC, CD45R, B220, T200, GP180
- Isotype
- Mouse IgG1, λ
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Human peripheral blood lymphocytes were stained with anti-human CD45RA FITC and anti-human CD45RO (clone S19021B) APC/Fire™ 750 anti-human (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right). Data shown were from CD3+ population.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 376805 | 25 tests | $155.00 | |||
| 376806 | 100 tests | $340.00 | |||
CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic human CD45 peptide spanning the junction region between exons 3 and 7.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The S19021B antibody can be used in combination with antibodies against CD45RA to discern memory and naïve T cells. Staining profile by S19021B on human peripheral blood lymphocyte is comparable to that by clone UCHL1.
Staining signal by clone S19021B on target cells can be completely blocked by pre-incubation with clone UCHL1; staining of UCHL1 on target cells can be partially blocked by pre-incubation with clone S19021B.
Staining signal by clone S19021B can be significantly increased on the cells after neuraminidase treatment, suggesting that S19021B binding epitope is likely affected by glycosylation. - RRID
-
AB_3083367 (BioLegend Cat. No. 376805)
AB_3083367 (BioLegend Cat. No. 376806)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
-
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Thomas M. 1989. Annu Rev Immunol. 7:339.
- Trowbridge I, et al. 1994. Annu Rev Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
Other Formats
View All CD45RO Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD45RO | S19021B | FC |
| APC/Fire™ 750 anti-human CD45RO | S19021B | FC |
| PE/Cyanine7 anti-human CD45RO | S19021B | FC |
| APC anti-human CD45RO | S19021B | FC |
| Spark PLUS B550™ anti-human CD45RO | S19021B | FC |
| PE/Dazzle™ 594 anti-human CD45RO | S19021B | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 750 anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS B550™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Dazzle™ 594 anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu...