PE/Cyanine7 anti-human CD45RO Antibody

Pricing & Availability
Clone
S19021B (See other available formats)
Regulatory Status
RUO
Other Names
PTPRC, CD45R, B220, T200, GP180
Isotype
Mouse IgG1, λ
S19021B_PECyanine7_CD45RO_Antibody_120423
Human peripheral blood lymphocytes were stained with anti-human CD45RA FITC and anti-human CD45RO (clone S19021B) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown were from CD3+ population.
  • S19021B_PECyanine7_CD45RO_Antibody_120423
    Human peripheral blood lymphocytes were stained with anti-human CD45RA FITC and anti-human CD45RO (clone S19021B) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown were from CD3+ population.
Compare all formats See PE/Cyanine7 spectral data
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376807 25 tests $145.00
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376808 100 tests $305.00
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Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic human CD45 peptide spanning the junction region between exons 3 and 7.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The S19021B antibody can be used in combination with antibodies against CD45RA to discern memory and naïve T cells. Staining profile by S19021B on human peripheral blood lymphocyte is comparable to that by clone UCHL1.

Staining signal by clone S19021B on target cells can be completely blocked by pre-incubation with clone UCHL1; staining of UCHL1 on target cells can be partially blocked by pre-incubation with clone S19021B.

Staining signal by clone S19021B can be significantly increased on the cells after neuraminidase treatment, suggesting that S19021B binding epitope is likely affected by glycosylation.

RRID
AB_3083368 (BioLegend Cat. No. 376807)
AB_3083368 (BioLegend Cat. No. 376808)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution

Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes

Function
Enhances TCR and BCR signaling
Cell Type
B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu Rev Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu Rev Immunol. 12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RO on UniProt.org
Go To Top Version: 1    Revision Date: 10/02/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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