Biotin anti-human CD38 Antibody anti-CD38

Pricing & Availability

Clone: HIT2 (See other available formats)
Regulatory Status: RUO
Workshop: III 155
Other Names: T10, ADP-ribosyl cyclase
Isotype: Mouse IgG1, κ

HIT2_Biotin_062507 — Human peripheral blood lymphocytes stained with biotinylated HIT2, followed by Sav-PE

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Cat #

Size

Price

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303518

100 µg

$94.00

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Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Horse, Cow

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶ and spatial biology (IBEX)^10,11.

Application References (PubMed link indicates BioLegend citation)

Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Dieu M. 1998. J. Exp. Med. 188:373.
Esser M, et al. 2001. J. Virol. 75:6173.
Jeannin P, et al. 1999. J. Immunol. 162:2044.
Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107.
van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC)
Bende RJ, et al. 2003. Am. J. Pathol. 162:105.
Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Cortez-Retamozo V, et al. 2012. Proc Natl Acad Sci U S A. 109:2491. PubMed
Lu E, et al. 2019. Nature. 567:244. PubMed
Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed

RRID

AB_2259861 (BioLegend Cat. No. 303518)

Antigen Details

Structure: ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution: T cells, B cells, NK, myeloid, plasma, and dendritic cells
Function: Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor: CD31, hyaluronic acid
Cell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469.
Gene ID: 952 View all products for this Gene ID
UniProt: View information about CD38 on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Other Formats

View All CD38 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD38	HIT2	FC
FITC anti-human CD38	HIT2	FC
PE anti-human CD38	HIT2	FC
PE/Cyanine5 anti-human CD38	HIT2	FC
Purified anti-human CD38	HIT2	FC,CyTOF®,IHC-F
Alexa Fluor® 488 anti-human CD38	HIT2	FC
Alexa Fluor® 647 anti-human CD38	HIT2	FC
PE/Cyanine7 anti-human CD38	HIT2	FC
Biotin anti-human CD38	HIT2	FC
PerCP anti-human CD38	HIT2	FC
PerCP/Cyanine5.5 anti-human CD38	HIT2	FC
Alexa Fluor® 700 anti-human CD38	HIT2	FC,SB
Brilliant Violet 421™ anti-human CD38	HIT2	FC
Brilliant Violet 711™ anti-human CD38	HIT2	FC
Brilliant Violet 785™ anti-human CD38	HIT2	FC
Brilliant Violet 605™ anti-human CD38	HIT2	FC
APC/Cyanine7 anti-human CD38	HIT2	FC
Purified anti-human CD38 (Maxpar® Ready)	HIT2	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD38	HIT2	FC
PE anti-human CD38	HIT2	FC
Brilliant Violet 510™ anti-human CD38	HIT2	FC
FITC anti-human CD38	HIT2	FC
PE/Dazzle™ 594 anti-human CD38	HIT2	FC
TotalSeq™-A0389 anti-human CD38	HIT2	PG
TotalSeq™-C0389 anti-human CD38	HIT2	PG
APC/Fire™ 750 anti-human CD38	HIT2	FC
TotalSeq™-B0389 anti-human CD38	HIT2	PG
APC/Fire™ 810 anti-human CD38	HIT2	FC
Spark NIR™ 685 anti-human CD38 Antibody	HIT2	FC
TotalSeq™-D0389 anti-human CD38	HIT2	PG
PerCP/Cyanine5.5 anti-human CD38	HIT2	FC
APC anti-human CD38	HIT2	FC
APC/Fire™ 750 anti-human CD38	HIT2	FC
PE/Cyanine7 anti-human CD38	HIT2	FC
GMP PE anti-human CD38	HIT2	FC
GMP FITC anti-human CD38	HIT2	FC
Pacific Blue™ anti-human CD38	HIT2	FC
Pacific Blue™ anti-human CD38	HIT2	FC
GMP APC/Fire™ 750 anti-human CD38	HIT2	FC
GMP PE/Dazzle™ 594 anti-human CD38	HIT2	FC
GMP APC anti-human CD38	HIT2	FC
GMP PE/Cyanine7 anti-human CD38	HIT2	FC
Spark Blue™ 515 anti-human CD38	HIT2	FC
GMP Pacific Blue™ anti-human CD38	HIT2	FC
Spark Violet™ 423 anti-human CD38	HIT2	FC
Brilliant Violet 650™ anti-human CD38	HIT2	FC
Spark PLUS B550™ anti-human CD38	HIT2	FC

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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