Biotin anti-human IL-1β Antibody

Pricing & Availability
Clone
H1b-98 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1β, H1, OAF, Catabolin, IFNb-inducing factor(il-1b,il1b)
Isotype
Mouse IgG2b, κ
H1b-98_Biotin_IL1b_Antibody_IF_100412
Human PBMCs, stimulated with 1 µg/ml of LPS for 8 h and treated with Brefeldin A during the last 4 h, were prepared by cytospin, fixed and permeabilized on a slide and then treated with endogenous biotin blocking kit (Vector labs). Slides were stained with anti-human IL-1β biotin (clone H1b-98) and DyLight™ 649 streptavidin (green) and counterstained with DAPI (blue). Images were acquired with an Olympus FV10i confocal microscope. Images courtesy of Teresa Rodriguez, Darius Schneider, and Matthias von Herrath, LaJolla Institute for Allergy and Immunology.
  • H1b-98_Biotin_IL1b_Antibody_IF_100412
    Human PBMCs, stimulated with 1 µg/ml of LPS for 8 h and treated with Brefeldin A during the last 4 h, were prepared by cytospin, fixed and permeabilized on a slide and then treated with endogenous biotin blocking kit (Vector labs). Slides were stained with anti-human IL-1β biotin (clone H1b-98) and DyLight™ 649 streptavidin (green) and counterstained with DAPI (blue). Images were acquired with an Olympus FV10i confocal microscope. Images courtesy of Teresa Rodriguez, Darius Schneider, and Matthias von Herrath, LaJolla Institute for Allergy and Immunology.
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511703 50 µg $118.00
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511704 500 µg $300.00
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. 

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.  Clone H1b-98 is useful as the detection antibody in a sandwich ELISA when used in conjunction with the purified H1b-27 antibody (Cat. No. 511601 or 511604). For ELISA detection applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 31.3 pg/mL are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2.0 - 8.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Haug V, et al. 2020. Transplantation. e260:104. PubMed
  2. Croden J, et al. 2021. PLoS One. 16:e0258208. PubMed
  3. Bando H, et al. 2019. Front Cell Infect Microbiol. 9:140. PubMed
  4. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  5. Watkins H, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.010. PubMed
RRID
AB_1236449 (BioLegend Cat. No. 511703)
AB_1236449 (BioLegend Cat. No. 511704)

Antigen Details

Structure
Cytokine; 17.3 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cell Targets
B cells, T cells, monocytes
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org
Go To Top Version: 5    Revision Date: 10/29/2021

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