Biotin anti-mouse CD9 Antibody

Pricing & Availability
Clone
MZ3 (See other available formats)
Regulatory Status
RUO
Other Names
DRAP-27, MRP-1, p-24
Isotype
Rat IgG2a, κ
A_MZ3_Biotin_061609.jpg
C57BL/6 bone marrow cells double stained with MZ3 biotin (Lower panel) or rat IgG2a isotype control (Upper panel) followed by Sav-PE, and anti-mouse Ly-6G/Ly-6C (RB6-8C5) FITC.
  • A_MZ3_Biotin_061609.jpg
    C57BL/6 bone marrow cells double stained with MZ3 biotin (Lower panel) or rat IgG2a isotype control (Upper panel) followed by Sav-PE, and anti-mouse Ly-6G/Ly-6C (RB6-8C5) FITC.
  • B_MZ3_Biotin_061609.jpg
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Cat # Size Price Quantity Check Availability
124803 50 µg $71.00
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124804 500 µg $293.00
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Description

CD9 is a surface glycoprotein of the tetraspannin family. It is expressed on a variety of cells, including nerve, muscle cells and many cells of hematopoietic origin. CD9 is found to participate in forming a large molecular cell complex with other membrane proteins, such as MHC class II, CD19, CD5 and other TM4SF molecules. It is reported that CD9 is a marker of marginal zone B cells, B1 cells and plasma cells. The diverse functions of CD9 may largely depend upon its associated molecules on different cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References

(PubMed link indicates BioLegend citation)
  1. Won WJ, et al. 2002. J. Immunol. 168:5605. (FC, WB)
Product Citations
  1. Ko J, et al. 2018. Lab Chip. 3.261805556. PubMed
  2. Hermann BP, et al. 2018. Cell Rep. 25:1650. PubMed
  3. La HM, et al. 2018. Nat Commun. 2.332638889. PubMed
  4. Suzuki S, et al. 2021. Cell Reports. 34(7):108752. PubMed
  5. Athman J, et al. 2015. J Immunol. 195: 1044-1053. PubMed
RRID
AB_2076036 (BioLegend Cat. No. 124803)
AB_2076036 (BioLegend Cat. No. 124804)

Antigen Details

Structure
24 kd surface glycoprotein belonging to the tetraspanin (TM4SF)family which is characterized by four transmembrane-spanning domains and two extracellular domains.
Distribution

CD9 is expressed in nerve, muscle, keratinocytes, fibroblasts and a variety of hematopoietic cells including monocytes, macrophages, granulocytes , platelets and activated T and B cells.

Interaction
CD9 may participate in forming large molecular complexes with other associated molecules.
Cell Type
B cells, Embryonic Stem Cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, Platelets, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Boucheix C, et al. 1991. J. Biol. Chem. 266:117
2. Lanza F, et al. 1991. J. Biol. Chem. 266:10638

Gene ID
12527 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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