- Clone
- BVD2-21C11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
- Isotype
- Rat IgG2a, κ
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PMA+ ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were surface stained with anti-human CD3 (clone UCHT1) PE. Cells were then fixed, permeabilized, then intracellularly stained with anti-human GM-CSF (clone BVD2-21C11) Brilliant Violet 421™ (left) or rat lgG2a, κ Brilliant Violet 421™ isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 502323 | 25 tests | $190.00 | |||
| 502324 | 100 tests | $390.00 | |||
Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant human GM-CSF.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: ELISA1-4 or ELISPOT3,4 Detection, Neutralization1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated5,6 and acetone-fixed7 frozen tissue sections, and immunocytochemistry.
Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended.
-
Application References
(PubMed link indicates BioLegend citation) -
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
- Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
- Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
- Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
- Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
- Andersson J, et al. 1994. Immunology 83:16. (IHC)
- Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)
- RRID
-
AB_3097305 (BioLegend Cat. No. 502323)
AB_3097305 (BioLegend Cat. No. 502324)
Antigen Details
- Structure
- Cytokine; 22 kD (Mammalian)
- Bioactivity
- Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
- Cell Sources
- T cells, macrophages, fibroblasts, endothelial cells, mast cells
- Cell Targets
- Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
- Receptors
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263. - Regulation
- Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
- Gene ID
- 1437 View all products for this Gene ID
- UniProt
- View information about GM-CSF on UniProt.org
Other Formats
View All GM-CSF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human GM-CSF | BVD2-21C11 | ELISA Detection,ELISPOT Detection,ICFC |
| PE anti-human GM-CSF | BVD2-21C11 | ICFC |
| Purified anti-human GM-CSF | BVD2-21C11 | ELISA Capture,ICFC,IP,WB |
| PerCP/Cyanine5.5 anti-human GM-CSF | BVD2-21C11 | ICFC |
| APC anti-human GM-CSF | BVD2-21C11 | ICFC |
| Pacific Blue™ anti-human GM-CSF | BVD2-21C11 | ICFC |
| Purified anti-human GM-CSF (Maxpar® Ready) | BVD2-21C11 | ELISA Capture,CyTOF® |
| PE/Dazzle™ 594 anti-human GM-CSF | BVD2-21C11 | ICFC |
| Ultra-LEAF™ Purified anti-human GM-CSF | BVD2-21C11 | Neut |
| TotalSeq™-B0943 anti-human GM-CSF | BVD2-21C11 | ICPG |
| Brilliant Violet 421™ anti-human GM-CSF | BVD2-21C11 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human GM-CSF
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PE anti-human GM-CSF
PMA and ionomycin-stimulated Th2 polarized human peripheral ... -
Purified anti-human GM-CSF
BVD2-21C11_PURE_GM-CSF_ELISA_092117 -
PerCP/Cyanine5.5 anti-human GM-CSF
PMA and ionomycin-stimulated Th2-polarized human peripheral ... -
APC anti-human GM-CSF
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Pacific Blue™ anti-human GM-CSF
PMA and ionomycin-stimulated Th2-polarized human peripheral ... 
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Purified anti-human GM-CSF (Maxpar® Ready)
Human PBMCs were incubated for 6 hours in media alone (top) ... 
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PE/Dazzle™ 594 anti-human GM-CSF
PMA and ionomycin-stimulated Th2-polarized human peripheral ... 
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Ultra-LEAF™ Purified anti-human GM-CSF
Recombinant human GM-CSF (Black Circles, Cat. No. 572904) i... -
TotalSeq™-B0943 anti-human GM-CSF
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Brilliant Violet 421™ anti-human GM-CSF
PMA+ ionomycin stimulated (6 hours) human peripheral blood l...