Brilliant Violet 421™ anti-mouse CD122 (IL-2Rβ) Antibody

Pricing & Availability
Clone
5H4 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2 Receptor β chain, IL-2Rβ
Isotype
Rat IgG2a, κ
5H4_BV421_CD122_Antibody_FC_100523
C57BL/6 mouse splenocytes were stained with anti-mouse NK1.1 FITC and anti-mouse CD122 (IL-2Rβ) (clone 5H4) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • 5H4_BV421_CD122_Antibody_FC_100523
    C57BL/6 mouse splenocytes were stained with anti-mouse NK1.1 FITC and anti-mouse CD122 (IL-2Rβ) (clone 5H4) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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105919 50 µg $235.00
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Description

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat myeloma YB2/0 transfected with truncated mouse Il-2Rβ cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Furse R, et al. 1993 Eur. J. Immunol. 23:3181. (IP)
  2. He YW, et al. 1995. J. Immunol. 154:1596. (IP)
  3. Liqons DL, et al. 2012. J Biol Chem. 287:34386. PubMed.
RRID
AB_3083102 (BioLegend Cat. No. 105919)

Antigen Details

Structure
Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution

T cells and B cells, NK cells, monocytes, macrophages

Function
Critical component of IL-2 and IL-15 signaling
Ligand/Receptor
IL-2, IL-15
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Gene ID
16185 View all products for this Gene ID
UniProt
View information about CD122 on UniProt.org
Go To Top Version: 1    Revision Date: 08/21/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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