Brilliant Violet 421™ anti-mouse CD16 Antibody

Pricing & Availability
Clone
S17014E (See other available formats)
Regulatory Status
RUO
Other Names
Fcgr3, Fc receptor, IgG, low affinity III, Fc gamma receptor, Fc gamma receptor 3
Isotype
Rat IgG2a, κ
S17014E_BV-421_CD16_FC_031224
C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.1 (clone S17016D) PE/Fire™ 700 and anti-mouse CD16 (clone S17014E) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • S17014E_BV-421_CD16_FC_031224
    C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.1 (clone S17016D) PE/Fire™ 700 and anti-mouse CD16 (clone S17014E) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability
158029 50 µg $270.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD16 also know as Fcgr3 is a 50-65 kD type I transmembrane protein, member of the Fc gamma receptor family and  Ig superfamily. CD16  is expressed  on most myeloid cells including monocytes, macrophages, dendritic cells, and is also expressed by NK cells and NKT cells. CD16 is involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC); its ligands are IgG1, IgG2a and IgG2b.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD16 - transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Anti-mouse CD16/32 clones 93 and 2.4G2 both block clone S17014E staining.

RRID
AB_3106103 (BioLegend Cat. No. 158029)

Antigen Details

Structure
Type I transmembrane protein, 50-65 kDa; member of the Fc gamma receptor family and Ig superfamily
Distribution

Expressed on most myeloid cells including monocytes, macrophages, dendritic cells; also on NK cells and NKT cells

Function
Fc receptor, involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Ligand/Receptor
IgG1, IgG2a and IgG2b
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Nimmerjahn F1 & Ravetch JV. 2008. Nat Rev Immunol. 8(1):34-47.
  2. Biburger M & Nimmerjahn F. 2012. Immunol Lett. 143(1):53-9.
  3. Arase N, et al. 2003. J Immunol. 170:3054.
Gene ID
14131 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org
Go To Top Version: 1    Revision Date: 03/12/2024

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account