Purified anti-mouse CD16 Antibody

Pricing & Availability
Clone
S17014E (See other available formats)
Regulatory Status
RUO
Other Names
Fcgr3, Fc receptor, IgG, low affinity III, Fc gamma receptor, Fc gamma receptor 3
Isotype
Rat IgG2a, κ
a-S17014E_PURE_CD16_Antibody_1_082319
C57BL/6 mouse splenocytes were stained with NK1.1 Brilliant Violet 785™ and purified CD16 (clone S17014E, left) or purified rat IgG2a, κ (right), followed by anti-rat IgG Alexa Fluor® 647.
  • a-S17014E_PURE_CD16_Antibody_1_082319
    C57BL/6 mouse splenocytes were stained with NK1.1 Brilliant Violet 785™ and purified CD16 (clone S17014E, left) or purified rat IgG2a, κ (right), followed by anti-rat IgG Alexa Fluor® 647.
  • b-S17014E_PURE_CD16_Antibody_2_082319
    C57BL/6 mouse bone marrow were stained with purified CD16 (clone S17014E, filled histogram) or purified rat IgG2a, κ (open histogram), followed by anti-rat IgG Alexa Fluor® 647. Data shown was gated on the myeloid cell population.
  • c-
    RBL-1 cells transfected with mouse A) CD16, B) CD32 or C) CD64 were stained with the following antibodies: Anti-mouse CD16 clone S17014E, Anti-mouse CD32 clone S17012B and Anti-mouse CD64 clone S18017D to confirm their specificity (filled histograms). Open histograms shows the corresponding isotype control.
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Cat # Size Price Quantity Check Availability
158002 500 µg $270.00
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Description

CD16 also know as Fcgr3 is a 50-65 kD type I transmembrane protein, member of the Fc gamma receptor family and  Ig superfamily. CD16  is expressed  on most myeloid cells including monocytes, macrophages, dendritic cells, and is also expressed by NK cells and NKT cells. CD16 is involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC); its ligands are IgG1, IgG2a and IgG2b.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD16 - transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Clone S17014E does not cross-react with CD32 or CD64.

Application Notes

Anti-mouse CD16/32 clones 93 and 2.4G2 both block clone S17014E staining.

Product Citations
  1. Wang L, et al. 2021. J Cell Physiol. 236:4625. PubMed
RRID
AB_2814096 (BioLegend Cat. No. 158002)

Antigen Details

Structure
Type I transmembrane protein, 50-65 kDa; member of the Fc gamma receptor family and Ig superfamily
Distribution

Expressed on most myeloid cells including monocytes, macrophages, dendritic cells; also on NK cells and NKT cells

Function
Fc receptor, involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Ligand/Receptor
IgG1, IgG2a and IgG2b
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Nimmerjahn F1 & Ravetch JV. 2008. Nat Rev Immunol. 8(1):34-47.
  2. Biburger M & Nimmerjahn F. 2012. Immunol Lett. 143(1):53-9.
  3. Arase N, et al. 2003. J Immunol. 170:3054.
Gene ID
14131 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org
Go To Top Version: 3    Revision Date: 08/04/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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