Brilliant Violet 421™ anti-mouse CD20 Antibody

Pricing & Availability
Clone
SA275A11 (See other available formats)
Regulatory Status
RUO
Other Names
Ms4a1, Ly-44
Isotype
Rat IgG2b, κ
1_SA275A11_BV421_CD20_Antibody_1_121115
C57BL/6 mouse splenocytes were stained with CD19 APC and CD20 (clone SA275A11) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 1_SA275A11_BV421_CD20_Antibody_1_121115
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD20 (clone SA275A11) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 2_SA275A11_BV421_CD20_Antibody_2_121115
  • 3_SA275A11_BV421_CD20_Antibody_IHCF_070517
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for one hour at room temperature. Then the section was stained with 2.5 µg/ml of anti-mouse CD20 (clone SA275A11) Brilliant Violet 421™ (blue) and 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 594 (red) at 4°C overnight. The image was captured with a 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data See high resolution IHC-F data...
Cat # Size Price Quantity Check Availability
150405 50 µg $292.00
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Description

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD20 - transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemical staining of frozen sections, the suggested use of this reagent is 2.5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Blanc P, et al. 2016. Nat Commun. 7:13600. PubMed
RRID
AB_2566540 (BioLegend Cat. No. 150405)

Antigen Details

Structure
Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution

B cells and a subset of T cells.

Function
Regulates B-cell activation and proliferation.
Interaction
Associated with MHC class I and II, CD53, CD81, and CD82.
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236.
3. Beers SA, et al. 2010. Blood 115:5191.
4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214.

Gene ID
12482 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org
Go To Top Version: 2    Revision Date: 06/17/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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