- Clone
- W18349C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcRI
- Isotype
- Rat IgG2a, κ
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C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC/Cyanine7 and anti-mouse CD64 (FcγRI) (clone W18349C) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right). Data shown was gated on lymphoid and myeloid population.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 164407 | 50 µg | $285.00 | |||
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse FcƳRI/CD64 (aa 25-297)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- RRID
-
AB_3068163 (BioLegend Cat. No. 164407)
Antigen Details
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Tan PS, et al. 2003. J Immunol. 170:2549. (IP)
- Ingersoll MA, et al. 2010. Blood. 115:e10. (FC)
- Ozeri E, et al. 2012. J Immunol. 189:146.
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825.
- Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
Other Formats
View All CD64 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD64 (FcγRI) | W18349C | FC |
| PE anti-mouse CD64 (FcγRI) | W18349C | FC |
| APC anti-mouse CD64 (FcγRI) | W18349C | FC |
| FITC anti-mouse CD64 (FcγRI) | W18349C | FC |
| Brilliant Violet 421™ anti-mouse CD64 (FcγRI) | W18349C | FC |
| PE/Dazzle™ 594 anti-mouse CD64 (FcγRI) | W18349C | FC |
| PE/Cyanine7 anti-mouse CD64 (FcγRI) | W18349C | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
APC anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
FITC anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Brilliant Violet 421™ anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Dazzle™ 594 anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Cyanine7 anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse...