Brilliant Violet 421™ anti-mouse GM-CSF Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Yeast-expressed, recombinant mouse GM-CSF

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

ELISA or ELISPOT Capture^1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry⁸: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.
Neutralization^2-4: The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^1,6,7, and immunocytochemistry⁸.

Application References

(PubMed link indicates BioLegend citation)

1. Sander B, et al. 1993. J. Immunol. Methods 166:201.
2. Suda T, et al. 1990. Cell. Immunol. 129:228.
3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
6. Sander B, et al. 1991. Immunol. Rev. 119:65.
7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
8. Larkin J, et al. 2006. J. Immunol. 177:268.

RRID

AB_3662370 (BioLegend Cat. No. 505427)

Antigen Details

Structure

Cytokine; 22 kD (Mammalian)

Bioactivity

Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors

Cell Sources

T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells

Cell Targets

Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts

Receptors

Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R

Biology Area

Cell Biology, Stem Cells

Molecular Family

Cytokines/Chemokines, Growth Factors

Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.

Regulation

Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans

Gene ID

12981 View all products for this Gene ID

UniProt

View information about GM-CSF on UniProt.org

Related Protocols

• Intracellular Flow Cytometry Staining Protocol

Other Formats