Brilliant Violet 421™ anti-TIF1β (KAP-1, TRIM28) Antibody

Pricing & Availability
Clone
20A1 (See other available formats)
Regulatory Status
RUO
Other Names
Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1
Isotype
Mouse IgG1, κ
20A1_BV421_TIF1beta_Antibody_1_062519
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 5 µg/ml of anti-TIF1β (clone 20A1) Brilliant Violet 421™ (blue) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DRAQ7™ (red). The image was captured with 40X objective.
  • 20A1_BV421_TIF1beta_Antibody_1_062519
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 5 µg/ml of anti-TIF1β (clone 20A1) Brilliant Violet 421™ (blue) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DRAQ7™ (red). The image was captured with 40X objective.
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Cat # Size Price Quantity Check Availability
619310 50 µg $264.00
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Description

TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

This clone may produce increased background by western blot when used at concentrations exceeding 0.1 µg/mL.

Application References

(PubMed link indicates BioLegend citation)
  1. Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)
RRID
AB_2616773 (BioLegend Cat. No. 619310)

Antigen Details

Structure
Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD
Distribution

Nuclear, associates with specific chromatin regions

Function
Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366.
2. Schultz DC, et al. 2002. Genes Dev. 16:919.
3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859.
4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
5. Messerschmidt DM, et al. 2012. Science 335:1499.
6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.

 

Gene ID
10155 View all products for this Gene ID
UniProt
View information about TIF1beta on UniProt.org
Go To Top Version: 1    Revision Date: 05/13/2016

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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