Brilliant Violet 570™ anti-human CD20 Antibody

Pricing & Availability
Clone
2H7 (See other available formats)
Regulatory Status
RUO
Workshop
IV B201
Other Names
B1, Bp35
Isotype
Mouse IgG2b, κ
2H7_BV570_091611
Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 570™ (filled histogram), or mouse IgG2b, κ Brilliant Violet 570™ (dashed line).
  • 2H7_BV570_091611
    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 570™ (filled histogram), or mouse IgG2b, κ Brilliant Violet 570™ (dashed line).
Compare all formats See Brilliant Violet 570™ spectral data
Cat # Size Price Quantity Check Availability
302331 25 tests $215.00
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302332 100 tests $435.00
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human tonsillar B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations
  1. Sun C, et al. 2022. Nat Biomed Eng. 6:1004. PubMed
  2. Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
  3. Nganou-Makamdop K, et al. 2021. Cell. 184(15):3899-3914.e16. PubMed
  4. Pauthner MG, et al. 2019. Immunity. 50:241. PubMed
  5. Gagne M, et al. 2022. Cell. 185:113. PubMed
  6. Harrington WE, et al. 2021. Cell Reports Medicine. 2(4):100253. PubMed
  7. Shangguan S, et al. 2021. Elife. 10:. PubMed
  8. Manuzak J, et al. 2016. J Immunol. 196: 2401 - 2409. PubMed
  9. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  10. Smedley J, et al. 2016. PLoS One. 11: 0157535. PubMed
  11. Amodio D, et al. 2018. J Immunol. 200:538. PubMed
  12. Raabe V, et al. 2020. Front Microbiol. 11:561530. PubMed
  13. Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
  14. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  15. Stone D, et al. 2021. Hum Gene Ther. 32:96. PubMed
  16. Fisher BS, et al. 2020. PLoS One. 15:e0233577. PubMed
  17. Darrah PA, et al. 2019. NPJ Vaccines. 4:21. PubMed
  18. Moysi E, et al. 2021. Front Immunol. 12:683396. PubMed
RRID
AB_2563805 (BioLegend Cat. No. 302331)
AB_2563805 (BioLegend Cat. No. 302332)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Other Formats

View All CD20 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD20 2H7 FC
FITC anti-human CD20 2H7 FC
PE anti-human CD20 2H7 FC
PE/Cyanine5 anti-human CD20 2H7 FC
Purified anti-human CD20 2H7 FC,CyTOF®,IHC-F,IP
APC/Cyanine7 anti-human CD20 2H7 FC
PE/Cyanine7 anti-human CD20 2H7 FC
Alexa Fluor® 488 anti-human CD20 2H7 FC,IHC-F
Alexa Fluor® 647 anti-human CD20 2H7 FC,IHC-F
Pacific Blue™ anti-human CD20 2H7 FC
Alexa Fluor® 700 anti-human CD20 2H7 FC
PerCP anti-human CD20 2H7 FC
PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Brilliant Violet 421™ anti-human CD20 2H7 FC,IHC-F
Brilliant Violet 570™ anti-human CD20 2H7 FC
Brilliant Violet 605™ anti-human CD20 2H7 FC
Brilliant Violet 650™ anti-human CD20 2H7 FC
Brilliant Violet 785™ anti-human CD20 2H7 FC
Brilliant Violet 510™ anti-human CD20 2H7 FC
Brilliant Violet 711™ anti-human CD20 2H7 FC
Purified anti-human CD20 (Maxpar® Ready) 2H7 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD20 2H7 FC
Biotin anti-human CD20 2H7 FC
APC/Fire™ 750 anti-human CD20 2H7 FC
Alexa Fluor® 594 anti-human CD20 2H7 ICC,IHC-F
FITC anti-human CD20 2H7 FC
Pacific Blue™ anti-human CD20 2H7 FC
APC anti-human CD20 2H7 FC
PE/Cyanine7 anti-human CD20 2H7 FC
TotalSeq™-A0100 anti-human CD20 2H7 PG
TotalSeq™-B0100 anti-human CD20 2H7 PG
TotalSeq™-C0100 anti-human CD20 2H7 PG
PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Spark NIR™ 685 anti-human CD20 2H7 FC
Spark YG™ 593 anti-human CD20 2H7 FC
GMP FITC anti-human CD20 2H7 FC
TotalSeq™-D0100 anti-human CD20 2H7 PG
GMP APC anti-human CD20 2H7 FC
Spark Violet™ 500 anti-human CD20 2H7 FC
GMP Pacific Blue™ anti-human CD20 2H7 FC
GMP PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Spark Violet™ 538 anti-human CD20 2H7 FC
APC/Fire™ 750 anti-human CD20 2H7 FC
PE anti-human CD20 2H7 FC
GMP PE/Cyanine7 anti-human CD20 2H7 FC
Spark Blue™ 515 anti-human CD20 2H7 FC
GMP APC/Fire™ 750 anti-human CD20 2H7 FC
GMP PE anti-human CD20 2H7 FC
Spark Blue™ 550 anti-human CD20 (Flexi-Fluor™) 2H7 FC
Spark Blue™ 574 anti-human CD20 (Flexi-Fluor™) 2H7 FC
Spark Red™ 718 anti-human CD20 (Flexi-Fluor™) 2H7 FC
APC/Fire™ 810 anti-human CD20 2H7 FC
Alexa Fluor® 660 anti-human CD20 2H7 FC
Go To Top Version: 3    Revision Date: 10/08/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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