Spark Blue™ 550 anti-human CD20 (Flexi-Fluor™) Antibody, CD20, 2H7

Pricing & Availability

Clone: 2H7 (See other available formats)
Regulatory Status: RUO
Workshop: IV B201
Other Names: B1, Bp35
Isotype: Mouse IgG2b, κ

Compare all formats See Spark Blue™ 550 spectral data

Cat #

Size

Price

Quantity

285006

50 µg

$394.00

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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca²⁺ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Technical data sheet

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human tonsillar B cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

Excitation Laser

Blue Laser (488 nm)

Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴ and immunohistochemical staining of acetone-fixed frozen sections⁵.

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
Polyak MJ, et al. 2002. Blood 99:3256. (IP)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)

RRID

AB_3106273 (BioLegend Cat. No. 285006)

Antigen Details

Structure: Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution: B cell, T cell subsets
Function: B cell activation
Ligand/Receptor: Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
Gene ID: 931 View all products for this Gene ID
UniProt: View information about CD20 on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Other Formats

View All CD20 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD20	2H7	FC
FITC anti-human CD20	2H7	FC
PE anti-human CD20	2H7	FC
PE/Cyanine5 anti-human CD20	2H7	FC
Purified anti-human CD20	2H7	FC,CyTOF®,IHC-F,IP
APC/Cyanine7 anti-human CD20	2H7	FC
PE/Cyanine7 anti-human CD20	2H7	FC
Alexa Fluor® 488 anti-human CD20	2H7	FC,IHC-F
Alexa Fluor® 647 anti-human CD20	2H7	FC,IHC-F
Pacific Blue™ anti-human CD20	2H7	FC
Alexa Fluor® 700 anti-human CD20	2H7	FC
PerCP anti-human CD20	2H7	FC
PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Brilliant Violet 421™ anti-human CD20	2H7	FC,IHC-F
Brilliant Violet 570™ anti-human CD20	2H7	FC
Brilliant Violet 605™ anti-human CD20	2H7	FC
Brilliant Violet 650™ anti-human CD20	2H7	FC
Brilliant Violet 785™ anti-human CD20	2H7	FC
Brilliant Violet 510™ anti-human CD20	2H7	FC
Brilliant Violet 711™ anti-human CD20	2H7	FC
Purified anti-human CD20 (Maxpar® Ready)	2H7	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD20	2H7	FC
Biotin anti-human CD20	2H7	FC
APC/Fire™ 750 anti-human CD20	2H7	FC
Alexa Fluor® 594 anti-human CD20	2H7	ICC,IHC-F
FITC anti-human CD20	2H7	FC
Pacific Blue™ anti-human CD20	2H7	FC
APC anti-human CD20	2H7	FC
PE/Cyanine7 anti-human CD20	2H7	FC
TotalSeq™-A0100 anti-human CD20	2H7	PG
TotalSeq™-B0100 anti-human CD20	2H7	PG
TotalSeq™-C0100 anti-human CD20	2H7	PG
PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Spark NIR™ 685 anti-human CD20	2H7	FC
Spark YG™ 593 anti-human CD20	2H7	FC
GMP FITC anti-human CD20	2H7	FC
TotalSeq™-D0100 anti-human CD20	2H7	PG
GMP APC anti-human CD20	2H7	FC
Spark Violet™ 500 anti-human CD20	2H7	FC
GMP Pacific Blue™ anti-human CD20	2H7	FC
GMP PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Spark Violet™ 538 anti-human CD20	2H7	FC
APC/Fire™ 750 anti-human CD20	2H7	FC
PE anti-human CD20	2H7	FC
GMP PE/Cyanine7 anti-human CD20	2H7	FC
Spark Blue™ 515 anti-human CD20	2H7	FC
GMP APC/Fire™ 750 anti-human CD20	2H7	FC
GMP PE anti-human CD20	2H7	FC
Spark Blue™ 550 anti-human CD20 (Flexi-Fluor™)	2H7	FC
Spark Blue™ 574 anti-human CD20 (Flexi-Fluor™)	2H7	FC
Spark Red™ 718 anti-human CD20 (Flexi-Fluor™)	2H7	FC
APC/Fire™ 810 anti-human CD20	2H7	FC
Alexa Fluor® 660 anti-human CD20	2H7	FC

Go To Top Version: 1 Revision Date: 02/06/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.