Brilliant Violet 605™ anti-mouse CD274 (B7-H1, PD-L1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse PD-L1-transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Application Notes

mAb MIH6 blocks the binding of mouse PD-L1 to PD-1 (CD279)

Application References

(PubMed link indicates BioLegend citation)

1. Gassner FJ, et al. 2015. Br J Haematol. 170:515 (Block)
2. Haile ST, et al. 2013. J Immunol. 191:2829 (FC)
3. Hirahara K, et al. 2012. Immunity. 36:1017 (Block)
4. Fife BT, et al. 2009. Nat Immunol. 10:1185 (Block)
5. Kanai T, et al. 2003. J Immunol. 171:4156 (Block)

Product Citations

1. Prasad S, et al. 2023. iScience. 26:106628. PubMed

RRID

AB_2814056 (BioLegend Cat. No. 153606)

Antigen Details

Structure

Type 1 transmembrane protein, member of the B7 family, 40kD.

Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Function

CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.

Ligand/Receptor

PD-1 (CD279)

Cell Type

B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells

Biology Area

Cancer Biomarkers, Costimulatory Molecules, Immunology

Molecular Family

Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Dorand RD. 2016. Science. 353:399.
2. Khan AR, et al. 2015. Nat Commun. 6:5997.
3. Kiyasu J, et al. 2015. Blood. 126:2193
4. Herold M, et al. 2015. J Immunol. 195:3584
5. Buddhisa S, et  al. 2015. J Immunol. 194:4413

Gene ID

60533 View all products for this Gene ID

UniProt

View information about CD274 on UniProt.org

Other Formats