Brilliant Violet 605™ anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
X54-5slash7.1_BV605_CD64_Antibody_1_082916
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-5slash7.1_BV605_CD64_Antibody_1_082916
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-5slash7.1_BV605_CD64_Antibody_2_082916
Compare all formats See Brilliant Violet 605™ spectral data
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139323 50 µg $292.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  2. Zaman R, et al. 2021. Immunity. :. PubMed
  3. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  4. Howard ZM, et al. 2022. Am J Physiol Cell Physiol. 322:C354. PubMed
  5. Roussel-Queval A, et al. 2023. STAR Protoc. 4:102119. PubMed
  6. Bhattacharjee A, et al. 2021. Immunity. 54(8):1745-1757.e7. PubMed
  7. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  8. Dong L, et al. 2021. Cancer Cell. . PubMed
  9. Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
  10. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  11. Warheit-Niemi HI, et al. 2022. JCI Insight. 7:. PubMed
  12. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  13. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  14. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  15. Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed
  16. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  17. Howard ZM, et al. 2022. JCI Insight. 7:. PubMed
  18. Sung PS, et al. 2022. JCI Insight. :. PubMed
  19. Molgora M, et al. 2020. Cell. 182:886. PubMed
  20. Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed
  21. Huang X, et al. 2021. Immunity. . PubMed
  22. Goc J, et al. 2021. Cell. 184:5015. PubMed
  23. Yang J, et al. 2022. Ann Transl Med. 10:1089. PubMed
  24. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  25. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  26. Trivedi S, et al. 2020. Elife. 9:00. PubMed
RRID
AB_2629778 (BioLegend Cat. No. 139323)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 2    Revision Date: 12/14/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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