Brilliant Violet 605™ anti-mouse CD86 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining of acetone-fixed frozen sections^2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses^1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References

(PubMed link indicates BioLegend citation)

1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed

Product Citations

1. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
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3. Tunali G, et al. 2023. J Clin Invest. :. PubMed
4. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
5. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
6. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
7. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
8. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
9. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
10. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
11. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
12. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
13. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
14. Duan L, et al. 2021. Immunity. 54:2256. PubMed
15. Kato Y, et al. 2020. Immunity. 53(3):548-563. PubMed
16. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
17. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
18. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
19. Liu D, et al. 2020. J Exp Med. 217:. PubMed
20. Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
21. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
22. Guilliams M, et al. 2022. Cell. 185:379. PubMed
23. Ishikura N, et al. 2022. Oncol Rep. 47:. PubMed
24. Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed
25. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
26. Škrnjug I, et al. 2014. PLoS One. 9:95728. PubMed
27. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
28. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
29. Hardy C, et al. 2013. J Immunol. 191:5278. PubMed
30. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
31. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
32. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed

RRID

AB_11204429 (BioLegend Cat. No. 105037)

Antigen Details

Structure

Ig superfamily, 80 kD

Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function

T cell costimulation, Ig class-switching, NK cell cytotoxicity

Ligand/Receptor

CD28, CD152 (CTLA-4)

Cell Type

Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs

Biology Area

Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID

12524 View all products for this Gene ID

UniProt

View information about CD86 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats