Brilliant Violet 650™ anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
6D5_BV650_051812
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 650™ (filled histogram) or rat IgG2a, κ Brilliant Violet 650™ isotype control (open histogram).
  • 6D5_BV650_051812
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 650™ (filled histogram) or rat IgG2a, κ Brilliant Violet 650™ isotype control (open histogram).
Compare all formats See Brilliant Violet 650™ spectral data
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115541 125 µL $209.00
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  2. Skladanowska K, et al. 2022. Cell Rep. 41:111490. PubMed
  3. Brunner SM, et al. 2023. Int J Mol Sci. 24:. PubMed
  4. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  5. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  6. Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed
  7. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  8. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  9. Kim EH, et al. 2020. Elife. 9:00. PubMed
  10. Matsushita Y, et al. 2021. J Bone Miner Res. 36:1145. PubMed
  11. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  12. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  13. Rojas OL et al. 2018. Cell. 176(3):610-624 . PubMed
  14. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  15. Al Nabhani Z, et al. 2019. Immunity. 50:1276. PubMed
  16. Zhuo M, et al. 2021. Pigment Cell Melanoma Res. 34:605. PubMed
  17. Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed
  18. McKee AM, et al. 2021. iScience. 24:103012. PubMed
  19. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  20. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  21. Wei SC, et al. 2020. Cancer Discov. . PubMed
  22. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  23. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  24. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  25. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  26. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  27. Tondini E, et al. 2022. NPJ Vaccines. 7:64. PubMed
  28. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  29. Parker KR, et al. 2020. Cell. 183(1):126-142.e17. PubMed
  30. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  31. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  32. Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
  33. Bruder J, et al. 2017. Mol Ther Methods Clin Dev. 10.1016/j.omtm.2017.08.003. PubMed
  34. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  35. Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed
  36. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  37. Aalipour A, et al. 2020. Mol Ther Oncolytics. 17:232. PubMed
  38. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  39. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
RRID
AB_11204087 (BioLegend Cat. No. 115541)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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